Physical Sciences and Mathematics Commons^™

Using Quantitative And Kinetic Proteomics To Explore Proteostasis, Nathan Raul Zuniga Pina

Theses and Dissertations

Every cell consists of carefully orchestrated biomolecules such as lipids, carbohydrates, and proteins. To maintain internal stability (homeostasis), cells maintain the right amount of these molecules at the right time and at the right place. This process is especially true for proteins since they are the foundation functional units within the cell. Proteins form structures and perform chemistry that bestows cells overarching functional roles. Cells maintain protein homeostasis (proteostasis) by modulating synthesis, folding, and degradation processes (turnover) to maintain the abundance levels for all proteins. This is the foundational kinetic model of proteostasis that is covered in this work, and …

The Synthesis And Optimization Of Sulfide And Halide Solid Electrolytes For All Solid-State Batteries, Teerth Brahmbhatt

Doctoral Dissertations

Countries and organizations around the world have established ambitious targets to transition away from fossil fuel-based energy sources and devices. The transition is focused on cleaning up power generation by converting coal, natural gas, and oil-based power generation to renewables and nuclear energy. Decarbonizing other sectors of energy use, transportation for example, will require broader electrification. To drive this move away from fossil fuel powered transportation will require portable energy storage devices. Conventional lithium-ion batteries are a popular candidate to lead this shift. However, these batteries often rely on flammable liquid electrolytes and carbon anodes that suffer from low energy …

Design, Synthesis And Immunological Testing Of Minimal Oligosaccharide Epitopes For Glycoconjugate Vaccines Targeting Capsular Polysaccharides Of Serotypes 3, 4, 7f And 9v Of Streptococcus Pneumoniae, Seth A. Taylor

Theses and Dissertations

The rise in antibiotic resistant strains of bacteria has led to the need for new methods of combatting bacterial infection. Since the surfaces of bacteria are covered in uniquely patterned capsular polysaccharides, vaccines targeting these polysaccharides have become a popular field of research for protection against pathogenic bacteria. Though licensed polysaccharide vaccines are commercially available, they lack the efficiency to protect patients that are immunocompromised or at high risk because they elicit T cell independent immune responses, resulting in low-affinity antibodies. To elicit a T cell dependent response and thereby recruit B cells that produce high-affinity antibodies, a vaccine was …

Design, Synthesis, And Kinetic Study Of The First Generation Phenyloxadiazole Compounds And The Second Generation Phenyloxadiazole Compounds And Highly Selective Hydroboration Of Alkenes, Ketones And Aldehydes Catalyzed By A Well-Defined Manganese Complex, Haisu Zeng

Dissertations, Theses, and Capstone Projects

This thesis focuses on two projects. In the first project, my goal was to discover a new variant of phenyloxadiazole compounds that could exhibit increased thiol-reactivity during bioconjugation processes. To achieve this, I synthesized several phenyloxadiazole derivatives and subjected them to NMR kinetic studies to assess their reactivity with a model thiol. Based on the results, I was able to identify a phenyloxadiazole analog that demonstrated enhanced reactivity, which included a chlorine atom located in the meta-position of the phenyl ring.

I provided a detailed description of the synthesis and characterization of the newly discovered phenyloxadiazole analog and demonstrate its …

Synthesizing, Purifying, And Characterizing Molten Chloride Salts, Phillip W. Halstenberg

Doctoral Dissertations

Molten chloride salts have vast potential as heat transfer fluids with both nuclear and concentrated solar power applications. For application in energy systems, the characteristics that govern these systems must be well understood. This work focuses on inorganic molten chloride salts with a special emphasis on the experimental aspect of chemical research. Chapter 2 covers the synthetic approaches for the formation of molten chloride mixtures. Many salts can be purchased from industrial suppliers, but most must be purified therefore, Chapter 3 evaluates various methodology developed for removal of impurities in salt mixtures. Once the salt of proper content and purity …

Synthesis, Radiolabeling And Evaluation Of A Suite Of Tracers With 44Sc For Detecting Extracellular Dna, Zhiyao Li

McKelvey School of Engineering Theses & Dissertations

Neutrophil extracellular traps involve the rapid translocation of DNA to the outside of the cell under certain stimuli. This structure forms a fibrous network that is able to limit the spread of pathogens and to kill microorganisms. It has also been shown to be present in various pathological processes such as inflammation, autoimmune diseases, and cancer metastasis. Currently, the formation process of NETs in vivo is being extensively studied. However noninvasive detection and quantitation has yet to be achieved. A class of PET tracers are described here that consists of a DNA dye as the backbone that is labeled with …

Synthesis And Characterization Of Rhenium 2,2′-Pyridylnaphthyridine Metal Complexes, Benjamin F. Solomon

Honors Theses

Rhenium (Re) is a group 7 heavy 5d transition metal in the same family as manganese (Mn) and technetium (Tc). Re-carbonyl complexes are known to be catalysts for many different kinds of reactions from C-C bond formation to reduction reactions like CO₂ reduction (CO₂RR)^1,2. With this in mind, we have prepared a set of six Re-carbonyl complexes of 2,2′-pyridyl-1,6-naphthyridine (1,6- pynap) and 2,2′-pyridyl-1,8-naphthyridine (1,8-pynap), a redox non-innocent ligand containing a bpy moiety as well as an additional aryl nitrogen binding location at which another metal-ligand bond could form. We have synthesized these complexes as precursors …

Utilizing Deep Learning Methods In The Identification And Synthesis Of Gene Regulations, Jiandong Wang

Theses and Dissertations

Gene expression is the fundamental differentiation and development process of life. Although all cells in an organism have essentially the same DNA, cell types and activities vary due to changes in gene expression. Gene expression can be influenced by many gene regulations. RNA editing contributes to the variety of RNA and proteins by allowing single nucleotide substitution. Reverse transcription can alter the expression status of genes by inducing genetic diversity and polymorphism via novel insertions, deletions, and recombination events. Gene regulation is critical to normal development because it enables cells to respond rapidly to environmental changes. However, identifying gene regulations …

Synthesis And Characterization Of Mixed Ligand Complexes Of Ruthenium(Ii) Containing 2,2’-Bipyridine And 3,3’-Dimethyl-1,1’- Methylenebisimidazolium Ligands; A New Synthetic Approach, Matthew Schneider

Theses and Dissertations

Research into solar cells has been prioritized given the global demand for better renewable energy technologies. This demand is a result of the long-term use of fossil fuels, which generates significant pollution in highly populated urban areas. One potential solar cell technology is the dye-sensitized solar cell (DSSC). This kind of solar technology utilizes organometallic dyes to expand the types of wavelengths solar cells can use to generate electricity.12 Ruthenium(II) metal complexes of this type have been investigated heavily for this purpose.13,14,36 The main reasoning behind this is the metal-to-ligand charge-transfer (MLCT) phenomenon that such complexes exhibit. MLCT has often …

Synthetic Methodologies Using Nucleophile-Electrophile Chemistry, Jacob C. Hood

Graduate Research Theses & Dissertations

Synthetic organic chemistry is a vital part of the modern world – making up 15-20% of the U.S. economy. New synthetic reactions can lower manufacturing costs and create more environmentally friendly processes. Substitution, addition, and superelectrophilic chemistry are potent tools to afford potentially essential compounds. The work outlined in this dissertation recounts the expansion of synthetic methodologies employing substrates with a wide range of electrophilicities toward synthesizing heterocyclic molecules. These concepts are summarized in chapter 1. Chapter 2: N-Heterocyclic alcohols are excellent substrates for superacid-promoted Friedel-Crafts reactions. The N-heterocyclic alcohol ionizes to produce reactive, dicationic intermediates, providing good to excellent …

Exploration Of Digital Synthesis, Angelo Indre

Williams Honors College, Honors Research Projects

“An Exploration of Digital Synthesis” is a comprehensive investigation into the world of digital audio and music production. The paper explores the fundamental concepts of sound synthesis, including MIDI, virtual instruments (VSTs), and the JUCE framework. The central focus of the paper is the implementation of a custom synthesizer, which serves as a case study for the practical application of digital synthesis. The paper addresses the key question of how to create a functioning synthesizer from scratch, providing detailed insights into the programming and design process. Overall, the paper represents a significant contribution to the fields of digital audio and …

Exploring The Role Of Ring Annulation In Polycyclic Aromatic Hydrocarbons For Organic Electronic Applications, Tanner Smith

Theses and Dissertations--Chemistry

Organic materials offer promising potential for the next generation of electronic devices, as their tunability, processability, and low-temperature manufacturing make them a cheap and versatile alternative to traditional silicon-based electronics. The ability to systematically alter the electronic properties of organic materials is vital for their incorporation into device applications. Polycyclic aromatic hydrocarbons (PAHs) are of significant interest for organic electronic applications, as relevant properties are highly dependent on their size, structure, and functionalities, and thus can be tuned to fit a wide variety of applications. Due to the enormous number of structural isomers available in larger PAHs, the development of …