Physical Sciences and Mathematics Commons^™

An Investigation Of The Oxygraphic Response Of Stressed Bovine Blood, Cristin Mckenna

Dissertations

Oxygraphic profiles of stressed bovine blood in a temperature controlled 2 ml reactor vessel provide the basis for an investigation of blood's response to various conditions. This response is broken down into an initial response (within 3 seconds), the response over 1 hour and the response over 24 hours. Fast-assay runs which investigate the initial response display a dose-response relationship when chemically stressed by phenol. Phenol concentrations in the 1,000 ppm range indicate a decrease in dissolved oxygen (DO) and higher phenol concentrations of over 20,000 an increase in DO. In addition to phenol concentration, other factors investigated with respect …

Oxidative Response Of Immobilized Bovine Blood To The Model Pollutant Phenol, Michael George Nickey

Theses

The primary purpose of this work is to determine if bovine blood can be studied in an immobilized cell bioreactor to determine if a phenol induced oxygen releasing optimum exists. This method of study has the potential to be used to determine what effects xenobiotics have on the oxidative activities of blood.

Three blood fractions containing approximately 21, 44, and 70% red blood cells (RBCs) were immobilized and placed in a recirculating bioreactor. These fractions were exposed injections of 0 ml, 3 ml, or 10 ml amounts of 2000 ppm phenol. The most active fraction was the 21% red cell …