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Full-Text Articles in Physical Sciences and Mathematics
State Of Bacterial Dna After Uptake Into The Roots Of Germinating Barley Seeds, Larry Dale Lawson
State Of Bacterial Dna After Uptake Into The Roots Of Germinating Barley Seeds, Larry Dale Lawson
Theses and Dissertations
Germinating barley seeds have been shown to take up Bacillus subtilis DNA labelled with 5-bromouracil and tritiated thymidine and retain the DNA in the root system without substantially degrading it. The percentage of bacterial DNA recovered from the total root DNA was independent of the amount of bacterial DNA available for uptake. Four observations indicated the stability of bacterial DNA after uptake into barley roots: (1) the bacterial DNA retained its transforming ability after recovery from the roots; (2) the labelled B. subtilis DNA showed similar CsCl density gradient patterns before uptake and after uptake; (3) most of the radioactivity …
Transforming Ability Of Bacillus Subtilis Dna Taken Up By Barley Embryos, Christine Tolman Ence
Transforming Ability Of Bacillus Subtilis Dna Taken Up By Barley Embryos, Christine Tolman Ence
Theses and Dissertations
Purified Bacillus subtilis DNA taken up by one-day-old barley embryos and permanently fixed within the barley cell is shown to have maintained its biological activity in B. subtilis transformation. Cesuim chloride density gradient centrifugation of DNA isolated from the roots of barley embryos after the uptake and incorporation of radioactive, bromouracil-labelled B. subtilis DNA indicates the presence of the bacterial DNA in several discreet bands of well-defined density, whose density is changed by sonication. These preliminary findings concerning the fate of bacterial DNA taken up by barley roots are discussed in terms of the recombination model of Ledoux and Huart …