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Full-Text Articles in Physical Sciences and Mathematics
Gas Phase Chiral Recognition, Characterization Of Porous Polymer Monolith Nanospray Ionization, And The Negative Mode Crafti Method Using Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, Nannan Fang
Theses and Dissertations
Our group has been studying chiral recognition in gas phase using mass spectrometry for more than 10 years. We are interested in gas phase studies of fundamental interactions because the gas phase avoids complications and masking effects that may arise upon solvation. Therefore, the results of gas phase experiments can be directly compared with those of high-level computational studies. In chapter 2, I studied the roles of hydrogen bonding and pi stacking in gas phase chiral recognition between aromatic crown molecules and aromatic amines. High affinity between host and guest doesn't necessarily result in better recognition. If the affinity is …
A Top-Down Proteomic Approach For The Discovery Of Novel Serum Biomarkers Of Pregnancy-Related Disease, Karen Merrell
A Top-Down Proteomic Approach For The Discovery Of Novel Serum Biomarkers Of Pregnancy-Related Disease, Karen Merrell
Theses and Dissertations
The serum fraction of blood is an ideal material in which to search for novel biomarkers for disease. It is easily obtained through relatively non-invasive means, routinely collected, and a rich treasure-trove of information about the health of an individual. Cells react to signal molecules, take up nutrients, and release waste products, fragments that are the result of proteolysis, and other molecules out into the bloodstream. If these components are unique to the cells in question, that part of the complex mixture that is the blood stream can potentially characterize the health of the tissue or organ those cells are …
Detection And Quantification Of Organophosphate Pesticides In Human Serum, Peter Kuklenyik
Detection And Quantification Of Organophosphate Pesticides In Human Serum, Peter Kuklenyik
Chemistry Dissertations
The United States Environmental Agency permits the use of 39 organophosphate pesticides. Many of these pesticides are acutely toxic and have lasting effect on human health. Organophosphates quickly metabolize in the body, therefore currently human exposure is studied by measuring the metabolic products in urine. In this work a suite of analytical methods was developed to determine the presence of un-metabolized organophosphate pesticides in human serum. First mass spectroscopic detection methods were evaluated. Gas chromatograph coupled tandem mass spectrometer was used to compare the detection limits using chemical and electron impact ionization. Positive chemical ionization was selected, because it provided …
Development Of Quantitative Bioanalytical Methods For The Measurement Of Pharmaceutical Compounds Via Hplc-Uv And Hplc-Ms/Ms, Melissa A. Mcculloch
Development Of Quantitative Bioanalytical Methods For The Measurement Of Pharmaceutical Compounds Via Hplc-Uv And Hplc-Ms/Ms, Melissa A. Mcculloch
ETD Archive
Research in pharmaceutical treatments for illnesses and disease processes has rapidly grown in the past decade. The field is fueled by advances in our understanding of the molecular mechanics of the human body, coupled with the development of sophisticated scientific instruments which can efficiently generate massive amounts of important data. This has led to an ever-growing need for the development of quantitative bioanalytical methods to measure pharmaceutical compounds. In this work, the theory behind the analytical processes and instruments that make these investigations possible is discussed. Further, the development of two high performance liquid chromatographic (HPLC) methods with both ultra …
Development Of A Novel Gradient Chromatofocusing Tandem Mass Spectometry Technique For The Determination Of Cationic Compounds In Biofluids;Identification Of Caspace 3 Cleavage Sites Of Nhe-1 By High Performance Liquid Chromatography- Mass Spectrometry, Jianhua Tang
ETD Archive
In Part I, work was done to develop a novel gradient chromatofocusing liquid chromatography-tandem mass spectrometry (MS/MS) technique for the determination of ionic compounds in biological matrix. The analysis of ionic compounds poses a great challenge to current LC-MS techniques, due to the fact that there is little or no retention. These ionic compounds are eluted at almost the same time as biological matrix, which is normally directed to waste prior to connecting to the mass spectrometer. A review of current LC-MS techniques for ionic compounds is given in Chapter 1. They all have advantages and disadvantages. To retain and …
Raman Spectroscopy Of Body Fluids : A Nondestructive Identification Technique For Forensic Applications, Kelly Virkler
Raman Spectroscopy Of Body Fluids : A Nondestructive Identification Technique For Forensic Applications, Kelly Virkler
Legacy Theses & Dissertations (2009 - 2024)
The analysis of body fluid evidence during forensic investigations is a complicated process, yet it is a critical step in determining the key facts of a crime. There is no doubt that DNA analysis is an extremely useful tool that can identify a suspect or victim, but crime scene investigators must first locate and identify potential body fluid stains that can be collected for DNA analysis. Current screening tests are destructive to the sample and can give false positive results, so a nondestructive technique such as Raman spectroscopy, that can possibly confirm the presence of a particular fluid, could lend …
Merged Electrospray Ionization Mass Spectrometry, Jianan Dong
Merged Electrospray Ionization Mass Spectrometry, Jianan Dong
LSU Doctoral Dissertations
Ambient ionization allows the analysis of materials in their native environment without sample preparation by creating ions outside the mass spectrometer. The goal of this research was to develop new ambient ionization techniques for application to solids, liquids and particles under ambient conditions. Samples to be analyzed were directly merged with an electrospray of droplets, and then ionized and detected by a mass spectrometer. An ion trap mass spectrometer was modified with a nanoelectrospray source and configured for three experiments: merged electrospray ionization of dry particles, merged electrospray ionization of combustion products, and infrared laser desorption/ablation using electrospray for post-ionization. …