Physical Sciences and Mathematics Commons^™

A Dialyzable Phospholipid Renin Inhibitor, Donna L. Webber

Senior Scholar Papers

Crude renin preparations from steer kidneys were incubated with Cd2+ and with Zn2+ elevation of renin activity resulted in both preparations. Analyses of each preparation against Tris-saline, and finally EDTA, removed the cations; however, renin activity remained elevated. An indirect activation of Renin by Cd2+ and, to a lesser extent, Zn2+, was thus demonstrated. Removal of the cations by Tris-saline and DTA-dialyses was suspected to involve the loss by dialysis of a renin inhibitor. An isolation technique for a proposed phospholipid renin inhibitor similar to bovine phosphatidylserine, reported by another laboratory, was thought to be unnecessarily arduous due to the …