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Articles 1 - 9 of 9
Full-Text Articles in Physical Sciences and Mathematics
Semen Quality And Chemical Oxidative Stress; Quantification And Remediation, Saleem Ali Bani-Hani
Semen Quality And Chemical Oxidative Stress; Quantification And Remediation, Saleem Ali Bani-Hani
ETD Archive
The necessary multi-step process to prepare human semen for assisted reproduction (AR) such as in vitro fertilization (IVF) is known to induce oxidative stress in human spermatozoa and subsequent damage to the integrity of their cell components and, thus, to their function. Literature reports have viewed this as a potential link with the high frequency of birth defects among the IVF babies. In this work, we studied the effect of incubation and centrifugation on human sperm quality using established AR protocols. We found that in vitro incubation and centrifugation (20 min at 220g) of sperm generally degrade sperm quality in …
Development Of Bioanalytical Methods For Clinical Applications And Drug Screening, Xiaohan Cai
Development Of Bioanalytical Methods For Clinical Applications And Drug Screening, Xiaohan Cai
ETD Archive
In the past decade, bioanalytical method development has become an integral part of clinical diagnosis, biomarker discovery, and drug discovery and development. The new and emerged bioanalytical technologies allow the quantitative and qualitative analysis of small molecules and biomolecules with high sensitivity and specificity. Specifically, the bioanalytical methods based on LC-MS and methylation-specific PCR are well suited for detecting low-abundance metabolites, proteins, and DNA in biofluids and tissues for biomarker investigation. They offer great clinical promises for early diseases diagnosis and therapeutic interventions. Besides, the LC-MS/MS quantitative method is essential for the estimation of pharmacokinetic and toxicological properties in drug …
Development Of Quantitative Lc-Ms/Ms Methods For The Pharmacological Studies Of Anti-Cancer Drugs, Lan Li
Development Of Quantitative Lc-Ms/Ms Methods For The Pharmacological Studies Of Anti-Cancer Drugs, Lan Li
ETD Archive
In the development of anti-cancer drugs, it is essential to study the pharmacological profiles of the drugs. Among the analytical tools utilized in the pharmacological studies, LC-MS/MS has gained increased popularity due to its unequivocal sensitivity and specificity, as well as the ability of handling a wide variety of compounds with relatively simple sample preparation procedures. In this work, a brief review on the method rational, instrumentations, analytical method validation, and work flow of the method development was included. The processes of LC-MS/MS method development for the pharmacological studies of three anti-cancer drugs (i.e., methoxyamine, fludarabine, and 6-benzylthioinosine) were illustrated. …
Capillary Gradient Chromatofocusing-Mass Spectrometry;A Sensitive Approach For Protein Analysis, James Anthony Hribar
Capillary Gradient Chromatofocusing-Mass Spectrometry;A Sensitive Approach For Protein Analysis, James Anthony Hribar
ETD Archive
Gradient chromatofocusing-mass spectrometry is a new technique for protein analysis recently introduced by our research group. Capable of separating and identifying proteins according to pI values and molecular weight, gradient chromatofocusing-mass spectrometry has been achieved by integrating a new ion-exchange chromatography technique called gradient chromatofocusing with a newly discovered buffer system that promotes mass spectrometry detection. Differing from traditional ion-exchange chromatography techniques, gradient chromatofocusing employs specific low molecular weight, volatile buffer components that are introduced onto an ion-exchange HPLC column by programming a binary gradient pumping system to deliver the correct proportions of acidic mobile phase to overcome buffering of …
Quantitative And Qualitative Mass Spectrometric Analysis Of Anticancer Agents, Drugs Of Abuse And Enzyme-Inhibitor Complexes, Keri M. Smith
Quantitative And Qualitative Mass Spectrometric Analysis Of Anticancer Agents, Drugs Of Abuse And Enzyme-Inhibitor Complexes, Keri M. Smith
ETD Archive
Mass spectrometry is a valuable tool in the analysis of many types of compounds. From small molecules to large proteins, mass spectrometry can help to interrogate samples and give insights to the origins of disease as well as calculate an exact concentration. Bioanalytical method development is an integral component in the measurement of such compounds. To facilitate proper analytical investigation, the process of method development, analysis, and interpretation must be understood. This work describes the basis and procedure for bioanalytical method development and its detailed application to preclinical studies of the antineoplastic agent hexamethylene bisacetamide and measurement ofillicit drugs of …
Thermal Analysis As An Important Research Tool For Colleges And Universities, Jeffrey Allen Fruscella
Thermal Analysis As An Important Research Tool For Colleges And Universities, Jeffrey Allen Fruscella
ETD Archive
Three experiments were performed to demonstrate that thermal analysis is an important tool for use in colleges and universities for conducting scientific research. The first experiment used thermogravimetry, differential scanning calorimetry and thermal mechanical analysis to compare polymer resins from two ResinKits®, one from 1994 and the other from 2010. Analysis was done to determine if resins from the 1994 kit were viable standards. The experiments showed significant thermal differences between select resins and it was concluded that resins form the 1994 ResinKit® are no longer acceptable as standards.The second experiment used thermogravimetry and differential scanning calorimetry to determine the …
Factors Affecting Heme Transfer From "Gapdh": A Possible Heme Carrier Protein, Rajesh Vempati
Factors Affecting Heme Transfer From "Gapdh": A Possible Heme Carrier Protein, Rajesh Vempati
ETD Archive
The aim of my research is to understand the mechanism of heme transfer by carrier proteins into heme proteins. The mechanism of heme insertion into proteins is poorly understood. Previous experiments reported from our lab identified GAPDH as a possible heme carrier protein involved in heme transfer (Ritu Chakravarti et al PNAS). This work focuses on 2 aspects of heme transfer. a.) Rate of transfer of heme from GAPDH to apomyoglobin. We examined the rate of transfer of native proto-heme and the analogue meso-heme. The results showed that the rate of heme transfer was twice as fast for meso-heme compared …
Novel Thermal Analytical Techniques To Characterize Drugs And Drug Delivery, Lakshmi Swetha Kaza
Novel Thermal Analytical Techniques To Characterize Drugs And Drug Delivery, Lakshmi Swetha Kaza
ETD Archive
This thesis encompasses three significant projects. The study includes the characterization and evaluation of the properties of a commercial contraceptive transdermal patch, Ortho Evra® by Dielectric Analysis and Differential Scanning Calorimetry (DSC). This study helps in monitoring the mobility of the drug and transport properties by Isothermal and Scanning Dielectric Analysis as a function of temperature and frequency. The drugs in this product are norelgestromin and ethinyl estradiol. DSC was used to detect any crystalline character of the drugs by their fusion properties. Having no melting endotherm and detecting a glass transition temperature suggested that the drugs in the patches …
Introduction To Pharmaceutical Thermal Analysis: A Teaching Tool, Shravan Singh Thakur
Introduction To Pharmaceutical Thermal Analysis: A Teaching Tool, Shravan Singh Thakur
ETD Archive
Significant Thermal Analysis-physical chemical data needs to be acquired by the new analyst whether an entry level chemist or a new function for the experienced pharmaceutical scientist. This teaching tool describes the introductory use of Differential Scanning Calorimetry (DSC), Thermogravimetric Analysis (TGA) and Thermomechanical Analysis (TMA) for characterizing pharmaceuticals. Optimum Experimental conditions for DSC, TGA and TMA will focus on collecting the best results and interpretations. Does the sample contain volatiles? Evaporation creates endothermic peaks, 2 water or solvent can lower the glass transition temperature (Tg) by up to 100°C and affect the crystallization temperature on cooling. The decomposition temperature …