Physical Sciences and Mathematics Commons^™

Ellipsometric Measurement Of Swelling Properties Associated With Ph Responsive Hydrogels In Solution, James F. Patton

Masters Theses

Previous investigations have demonstrated that aqueous solutions containing hydrogels using 2-hydroxyethyl methacrylate (2-HEMA) as one of the monomer components swell reversibly under variable conditions of pH, temperature, and ionic strength, based upon weight gain ratios. A more unique approach will be employed to measure swelling properties associated with pH responsive hydrogels in buffered solutions, namely ellipsometry. Traditionally ellipsometry has been used to measure thickness of materials on a nanoscale dimension under ambient conditions. Finally, more novel approaches of employing hydrogel swelling response properties will be investigated; including response to glucose and enzyme loading schemes. The goal of our project will …

Studies Of Reversible Inhibition, Irreversible Inhibition And Activation Of Alkaline Phosphatase By Capillary Electrophoresis, Angela Randall Whisnant

Masters Theses

Reversible inhibition, irreversible inhibition, and activation of calf intestinal alkaline phosphatase (EC 3.1.3.1) have been studied by capillary electrophoresis. The capillary electrophoretic enzyme-inhibitor assays were performed by electrophoretically mixing zones of inhibitor and enzyme in a substrate-filled capillary. Enzyme inhibition was indicated by a decrease in product formation detected in the capillary by laser-induced fluorescence. Reversible enzyme inhibitors could be quantified by Michaelis-Menten treatment of the electrophoretic data. Reversible, competitive inhibition of alkaline phosphatase by sodium vanadate and sodium arsenate has been examined. The calculated K_i value for the capillary electrophoretic enzyme-inhibitor assays was 2.1 µM for sodium vanadate …

Synthetic Studies On Anticancer Compounds: The Tylophorinines, Conrad Gracjan Kaczmarek

Masters Theses

This thesis describes synthetic studies in the area of alkaloid chemistry, specifically, potent anticancer compounds: the tylophorinines.

Tylophorinine is a naturally occurring compound found in the Monarch butterfly and plants of the genus Tylophora. Described here is a concise methodology for stereoselective synthesis of this compound, and analogs, starting from simple, naturally occurring compounds. The synthetic strategy represents a simplification in synthesizing tylophorinines, and would allow numerous other tylophorinines with similar chemical structure to be conveniently synthesized in an analogous fashion.

Further, the tylophorinines are convenient compounds for determining where a cancer cell may be most effectively attacked. Numerous …