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Full-Text Articles in Physical Sciences and Mathematics

Quantitative Field Testing Rotylenchulus Reniformis Dna From Metagenomic Samples Isolated Directly From Soil., Kurt Showmaker, Gary W. Lawrence, Shien Lu, Clarissa Balbalian, Vincent P. Klink Dec 2011

Quantitative Field Testing Rotylenchulus Reniformis Dna From Metagenomic Samples Isolated Directly From Soil., Kurt Showmaker, Gary W. Lawrence, Shien Lu, Clarissa Balbalian, Vincent P. Klink

College of Agriculture & Life Sciences Publications and Scholarship

A quantitative PCR procedure targeting the ?-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil.


Utilizing Service-Learning In The Analytical Chemistry Laboratory: Soil And Water Analysis In Rochester, New York, Kimberly D. Chichester, Irene Kimaru, Lynn Donahue, Maryann Herman Jul 2011

Utilizing Service-Learning In The Analytical Chemistry Laboratory: Soil And Water Analysis In Rochester, New York, Kimberly D. Chichester, Irene Kimaru, Lynn Donahue, Maryann Herman

Chemistry Faculty/Staff Publications

For an analytical chemistry course at St. John Fisher College, instructors designed a servicelearning project on soil and water analysis to achieve the following two goals: 1) to introduce analytical chemistry students to soil- and water-testing methods by working in collaboration with surrounding neighborhoods residents and government agencies and 2) to prepare written reports of the results for the designated community partners. Service-learning students conducted soil testing for lead on homes and perspective community garden sites around Rochester, NY with plans to establish planting methods to revitalize polluted soil. Four different communities contributed soil samples. The entire project was performed …


Formation, Adsorption, And Degradation Of N-Nitrosoatrazine In Water And Soil, Hsin-Ro Wei Apr 2011

Formation, Adsorption, And Degradation Of N-Nitrosoatrazine In Water And Soil, Hsin-Ro Wei

School of Natural Resources: Dissertations, Theses, and Student Research

The products of xenobiotic reactions in environmental mammalian systems may pose risks equal to or greater than parent compounds. Products of concern include nitrosamines, which can be carcinogenic, mutagenic, and teratogenic. Nitrosamines may form in soil, lake water, and sewage; they also can form in agricultural soils treated with agrichemicals containing amine moieties and nitrogen fertilizer. The nitrosamine-forming reaction of amines with nitrite is promoted at acidic pH. The widely used herbicide atrazine has secondary amine groups that can react with nitrite to form N-nitrosoatrazine (NNAT). The primary objective of this research was to characterize the formation, stability, and …


Cellulose- And Xylan-Degrading Thermophilic Anaerobic Bacteria From Biocompost, M. V. Sizova, J. A. Izquierdo, N. S. Panikov, L. R. Lynd Feb 2011

Cellulose- And Xylan-Degrading Thermophilic Anaerobic Bacteria From Biocompost, M. V. Sizova, J. A. Izquierdo, N. S. Panikov, L. R. Lynd

Dartmouth Scholarship

Nine thermophilic cellulolytic clostridial isolates and four other noncellulolytic bacterial isolates were isolated from self-heated biocompost via preliminary enrichment culture on microcrystalline cellulose. All cellulolytic isolates grew vigorously on cellulose, with the formation of either ethanol and acetate or acetate and formate as principal fermentation products as well as lactate and glycerol as minor products. In addition, two out of nine cellulolytic strains were able to utilize xylan and pretreated wood with roughly the same efficiency as for cellulose. The major products of xylan fermentation were acetate and formate, with minor contributions of lactate and ethanol. Phylogenetic analyses of 16S …


Numerical Evaluation Of Subsurface Soil Water Evaporation Derived From Sensible Heat Balance, Masaru Sakai, Scott B. Jones, Markus Tuller Feb 2011

Numerical Evaluation Of Subsurface Soil Water Evaporation Derived From Sensible Heat Balance, Masaru Sakai, Scott B. Jones, Markus Tuller

Plants, Soils, and Climate Faculty Publications

A recently introduced measurement approach allows in situ determination of subsurface soil water evaporation by means of heat-pulse probes (HPP). The latent heat component of subsurface evaporation is estimated from the residual of the sensible heat balance. This heat balance method requires measurement of vertical soil temperature and estimates of thermal properties for soil water evaporation determination. Our objective was to employ numerically simulated thermal and hydraulic processes using constant or diurnally cycled surface boundary conditions to evaluate and understand this technique. Three observation grid spacings, namely, 6 mm (tri-needle HPP), 3 mm (penta-needle HPP) and 1 mm, along with …


Distribution And Properties Of Vesicular Horizons In The Western United States, Judith K. Turk, Robert C. Graham Jan 2011

Distribution And Properties Of Vesicular Horizons In The Western United States, Judith K. Turk, Robert C. Graham

Conservation and Survey Division

Vesicular horizons are thin (usuallycm) surface or near-surface horizons characterized by the predominance of vesicular porosity. Th ey are widespread in arid and semiarid lands, occurring on every continent and covering 156,000 km2 of the western United States. Vesicular horizons have critical implications for management due to their role in controlling surface hydrology and dust mobilization. Th is study evaluates the distribution and varia-tion in expression of vesicular horizons across the western United States using the soil databases available from the USDA. A vesicular horizon index (VHI) that incorporates vesicular horizon thickness and the size and quantity of vesicular pores …