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Full-Text Articles in Physical Sciences and Mathematics
The Thioflavin T Fluorescence Assay For Amyloid Fibril Detection Can Be Biased By The Presence Of Exogenous Compounds, Sean A. Hudson, Heath Ecroyd, Tak W. Kee, John A. Carver
The Thioflavin T Fluorescence Assay For Amyloid Fibril Detection Can Be Biased By The Presence Of Exogenous Compounds, Sean A. Hudson, Heath Ecroyd, Tak W. Kee, John A. Carver
Heath Ecroyd
Thioflavin T (ThT) dye fluorescence is used regularly to quantify the formation and inhibition of amyloid fibrils in the presence of anti-amyloidogenic compounds such as polyphenols. However, in this study, it was shown, using three polyphenolics (curcumin, quercetin and resveratrol), that ThT fluorescence should be used with caution in the presence of such exogenous compounds. The strong absorptive and fluorescent properties of quercetin and curcumin were found to significantly bias the ThT fluorescence readings in both in situ real-time ThT assays and single time-point dilution ThT-type assays. The presence of curcumin at concentrations as low as 0.01 and 1 uM …
Detection And Quantification Of Tear Phospholipids And Cholesterol In Contact Lens Deposits: The Effect Of Contact Lens Material And Lens Care Solution, Jennifer Saville, Zhenjun Zhao, Mark D.P. Willcox, Stephen J. Blanksby, Todd W. Mitchell
Detection And Quantification Of Tear Phospholipids And Cholesterol In Contact Lens Deposits: The Effect Of Contact Lens Material And Lens Care Solution, Jennifer Saville, Zhenjun Zhao, Mark D.P. Willcox, Stephen J. Blanksby, Todd W. Mitchell
Stephen Blanksby
PURPOSE. To examine the deposition of tear phospholipids and cholesterol onto worn contact lenses and the effect of lens material and lens care solution. METHODS. Lipids were extracted from tears and worn contact lenses using 2: 1 chloroform: methanol and the extract washed with aqueous ammonium acetate, before analysis by electrospray ionization tandem mass spectrometry (ESI-MS/MS). RESULTS. Twenty-three molecular lipids from the sphingomyelin (SM) and phosphatidylcholine (PC) classes were detected in tears, with total concentrations of each class determined to be 5 +/- 1 pmol/mu L (similar to 3.8 mu g/mL) and 6 +/- 1 pmol/mu L (similar to 4.6 …
Automatic Detection Of Microcalcifications In Mammorgams Using A Fuzzy Classifier, Fazel Naghdy, Golshah Naghdy, Agustinus Drijarkara
Automatic Detection Of Microcalcifications In Mammorgams Using A Fuzzy Classifier, Fazel Naghdy, Golshah Naghdy, Agustinus Drijarkara
Professor Fazel Naghdy
No abstract provided.