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Effects Of Seasonal Variations And Heat Exposure On Antioxidant Expression And Redox Status In The American Oyster, Crassostrea Virginica: Field And Laboratory Studies, Md Sadequr Rahman
Effects Of Seasonal Variations And Heat Exposure On Antioxidant Expression And Redox Status In The American Oyster, Crassostrea Virginica: Field And Laboratory Studies, Md Sadequr Rahman
Theses and Dissertations
Increasing atmospheric temperatures influence physiological functions in aquatic organisms. In this study, I observed the effects of seasonal (field study) and laboratory heat exposure (16, 22, 26, and 30°C for 1-week) on prooxidant-antioxidant homeostasis in gills and digestive glands of American oyster. Histological analysis showed an increase in mucus secretion with elevated temperatures in both organs. Immunohistochemical analysis showed a significant increase of heat shock protein-70, dinitrophenyl, and 3-nitrotyrosine protein expressions with higher seasonal and laboratory temperatures indicating heat-induced oxidative and nitrosative stress. This was concomitant with gradual rise in cellular apoptosis. Interestingly, catalase and superoxide dismutase expressions also increased …
Detection And Enumeration Of Bacterial Pathogens In The American Oyster (Crassostrea Virginica), Mohammad Maruf Billah
Detection And Enumeration Of Bacterial Pathogens In The American Oyster (Crassostrea Virginica), Mohammad Maruf Billah
Theses and Dissertations
American oyster (Crassostrea virginica) is a popular seafood for its delicacy and high nutritional value. Based on increasing concern about contamination of bacterial pathogens in raw oyster, my research objectives have been focused on detection and enumeration of two important bacterial pathogens, Escherichia coli and Salmonella spp. in the American oyster in south Texas waters, local markets and controlled laboratory studies. Immunohistochemical and RT-PCR analyses showed substantial bacterial pathogen’s presence in gills and digestive glands of oysters collected from San Martin Lake and South Padre Island as well as local markets. Laboratory studies showed increasing trend of both …