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Assessment Of Energetic Heterogeneity Of Reversed-Phase Surfaces Using Excess Adsorption Isotherms For Hplc Column Characterization, Leih-Shan Yeung
Assessment Of Energetic Heterogeneity Of Reversed-Phase Surfaces Using Excess Adsorption Isotherms For Hplc Column Characterization, Leih-Shan Yeung
Seton Hall University Dissertations and Theses (ETDs)
This research is to explore a more general column categorization method using the test attributes in alignment with the common mobile phase components. As we know, the primary driving force for solute retention on a reversed-phase surface is hydrophobic interaction, thus hydrophobicity of the column will directly affect the analyte retention. This research describes a method to determine the column hydrophobicity by the ratio of adsorbed acetonitrile and methanol to water on the column surface using excess adsorption isotherm estimation. An excess adsorption isotherm for a binary mobile phase system represents a competitive interaction of both solvent components with the …