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Full-Text Articles in Physical Sciences and Mathematics
Imaging Stability In Force-Feedback High-Speed Atomic Force Microscopy, Byung I. Kim, Ryan Boehm
Imaging Stability In Force-Feedback High-Speed Atomic Force Microscopy, Byung I. Kim, Ryan Boehm
Physics Faculty Publications and Presentations
We studied the stability of force-feedback high-speed atomic force microscopy (HSAFM) by imaging soft, hard, and biological sample surfaces at various applied forces. The HSAFM images showed sudden topographic variations of streaky fringes with a negative applied force when collected on a soft hydrocarbon film grown on a grating sample, whereas they showed stable topographic features with positive applied forces. The instability of HSAFM images with the negative applied force was explained by the transition between contact and noncontact regimes in the force-distance curve. When the grating surface was cleaned, and thus hydrophilic by removing the hydrocarbon film, enhanced imaging …
Force-Feedback High-Speed Atomic Force Microscope For Studying Large Biological Systems, Byung I. Kim, Ryan Boehm
Force-Feedback High-Speed Atomic Force Microscope For Studying Large Biological Systems, Byung I. Kim, Ryan Boehm
Physics Faculty Publications and Presentations
We designed and developed a high-speed atomic force microscope (HSAFM) utilizing a force-feedback scheme for imaging large biological samples. The system collects three simultaneous images: a deflection image, a topographic image, and a force image. We demonstrated that this force-feedback HSAFM is capable of acquiring large topographic images of Escherichia coli biofilms at approximately one frame per second in air. We discuss how the self-actuating cantilever and the piezo tube follow those larger biological topographic features during the HSAFM imaging process.