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Expression, Identification, And Bioactivity Of Recombinant Human Erythropoietin From Lmh/2a (Leghorn Male Hepatoma)Cell Line, Amanda Cecile Broussard
Expression, Identification, And Bioactivity Of Recombinant Human Erythropoietin From Lmh/2a (Leghorn Male Hepatoma)Cell Line, Amanda Cecile Broussard
LSU Master's Theses
Erythropoietin (EPO) is the main cytokine regulator for red blood-cell production in the human by binding to erythropoietin receptors to promote erythroid proliferation, survival and differentiation. Administration of recombinant human EPO (rhEPO) as a therapeutic has been shown to improve renal and non-renal anemia and this success has led to a high medical global demand. Chinese hamster ovary (CHO) cells, yeast, and bacteria have been used to produce rhEPO but nevertheless there is still a need for a low-cost and high quality expression system for rhEPO. LMH/2A cells, as an alternative model, were transfected with the codon optimized human EPO …