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Full-Text Articles in Physical Sciences and Mathematics
Binding Of The Molecular Chaperone Alphab-Crystallin To Abeta Amyloid Fibrils Inhibits Fibril Elongation, Sarah L. Shammas, Christopher A. Waudby, Shuyu Wang, Alexander K. Buell, Tuomas P. Knowles, Heath W. Ecroyd, Mark E. Welland, John A. Carver, Christopher M. Dobson, Sarah Meehan
Binding Of The Molecular Chaperone Alphab-Crystallin To Abeta Amyloid Fibrils Inhibits Fibril Elongation, Sarah L. Shammas, Christopher A. Waudby, Shuyu Wang, Alexander K. Buell, Tuomas P. Knowles, Heath W. Ecroyd, Mark E. Welland, John A. Carver, Christopher M. Dobson, Sarah Meehan
Faculty of Science - Papers (Archive)
The molecular chaperone αB-crystallin is a small heat-shock protein that is upregulated in response to a multitude of stress stimuli, and is found colocalized with Aβ amyloid fibrils in the extracellular plaques that are characteristic of Alzheimer's disease. We investigated whether this archetypical small heat-shock protein has the ability to interact with Aβ fibrils in vitro. We find that αB-crystallin binds to wild-type Aβ42 fibrils with micromolar affinity, and also binds to fibrils formed from the E22G Arctic mutation of Aβ42. Immunoelectron microscopy confirms that binding occurs along the entire length …
Speciation Of Dissolved Inorganic Arsenic By Diffusive Gradients In Thin Films: Selective Binding Of Asiii By 3-Mercaptopropyl-Functionalized Silica Gel, William W. Bennett, Peter R. Teasdale, Jarad G. Panther, David T. Welsh, Dianne F. Jolley
Speciation Of Dissolved Inorganic Arsenic By Diffusive Gradients In Thin Films: Selective Binding Of Asiii By 3-Mercaptopropyl-Functionalized Silica Gel, William W. Bennett, Peter R. Teasdale, Jarad G. Panther, David T. Welsh, Dianne F. Jolley
Faculty of Science - Papers (Archive)
A diffusive gradients in thin films (DGT) technique for selectively measuring AsIII utilizes commercially available 3-mercaptopropyl- functionalized silica gel. Deployment of the new technique alongside the Metsorb-DGT for total inorganic arsenic allows the calculation of As III directly and AsV by difference. Uptake of As III by mercapto-silica was quantitative and elution with a mixture of 1 mol L-1 HNO3 and 0.01 mol L-1 KIO 3 gave a recovery of 85.6 + 1.7%. DGT validation experiments showed linear accumulation of AsIII over time (R2 > 0.998). Accumulation was unaffected by varying ionic strength (0.0001-0.75 mol L-1 NaNO3) and pH (3.5-8.5). Deployment …