Physical Sciences and Mathematics Commons^™

Natural Product Discovery And Mycotoxin Analysis Of Agricultural Endophytes And Pathogens, Jacob P. Walsh

Electronic Thesis and Dissertation Repository

This thesis focuses on the application of fungal natural product chemistry for the use of agriculture fungal pathogenesis research. The research in this work uses many analytical chemistry techniques and instruments such as HPLC, LC-MS/MS and NMR. In Chapter 2, a diagnostic fragmentation module was developed for the identification of compound classes in crude fungal extracts. This module identified the classes cytochalasins and chaetoglobosins from extracts of Penicillium, Chaetomium, and Xylaria. From these extracts a new chaetoglobosin was discovered and the module was implemented into the open-source software package MZmine 2.0. Chapter 3 discusses analytical chemistry techniques with the …