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Full-Text Articles in Physical Sciences and Mathematics
Characterization Of Highly Heterogeneous Heparin-Protein Complexes Using Novel Mass Spectrometry-Based Approaches, Yunlong Zhao
Characterization Of Highly Heterogeneous Heparin-Protein Complexes Using Novel Mass Spectrometry-Based Approaches, Yunlong Zhao
Doctoral Dissertations
Heparin-like glycosaminoglycan (GAG) is a family of polysaccharide involved in variety of physiological processes. They have potentials to interact with a broad range of proteins and many of them hold crucial values in regulation of protein functions. My dissertation addresses the significance and challenges in the field of heparin-mediated studies, with a focus on the questions in biological and analytical aspects, which are largely hindered by the structural heterogeneity and function diversity of heparin. My dissertation reports the efforts I made in the past few years with respect to the development of novel analytical strategies based on a combination of …
Analytical Methods To Support Design And Optimization Of Protein Drug Conjugate: Focusing On Haptoglobin-Hemoglobin Complex As A Drug Carrier, Shengsheng Xu
Doctoral Dissertations
Acquired immunodeficiency syndrome (AIDS) remains one of the most serious public health challenges and a significant cause of mortality for certain populations. Despite the large number of antiretrovirals (mostly nucleotide and nucleoside analogs) developed in the past two decades, the inability of small molecule therapeutics to target HIV reservoirs directly creates a significant obstacle to their effective utilization. Indeed, achieving the desired therapeutic effect in the absence of the effective targeted delivery must rely on dosage escalation, which frequently causes severe toxicity. This problem may be solved by conjugation of antiretroviral agents to endogenous proteins (e.g., hemoglobin haptoglobin complex) that …
New Mass Spectrometric Methods For Studying Proteins In Solution And In The Gas Phase, Zhe Zhang
New Mass Spectrometric Methods For Studying Proteins In Solution And In The Gas Phase, Zhe Zhang
Doctoral Dissertations
This dissertation focuses on the mass spectrometric based methods for studying protein structures in solution phase and in gas phase. Protein structure is important to study because it is related to many disease mechanism and related therapies. The relationship between protein structures in solution phase and in gas phase has been investigated. Especially, the electrostatic interaction is characterized by mass spectrometry (MS). A HDX method has been developed to study protein oligomer kinetics and applied to study β-2-microglobulin (β2m) oligomerization. This dissertation firstly focuses on the relationship between protein gas phase structure and solution phase structure. We studied the structures …
Analysis Of Gold Nanoparticles And Their Use With Laser Desorption/Ionization Mass Spectrometry, Alyssa Marsico
Analysis Of Gold Nanoparticles And Their Use With Laser Desorption/Ionization Mass Spectrometry, Alyssa Marsico
Doctoral Dissertations
Gold nanoparticles (AuNPs) have many unique properties that make them attractive for use in various biological applications. Laser desorption/ionization mass spectrometry (LDI-MS) has been used to monitor AuNPs in complex biological samples but there are still many ways in which AuNPs can be used with MS. In this dissertation, the use of AuNPs to assist in analyte ionization has been investigated. Their ability to enhance signal from biomolecules based on their surface chemistry, size, aggregation and method of deposition has been studied. The first use of an inkjet printer to create surfaces from which analytes can be sampled is discussed …