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Full-Text Articles in Physical Sciences and Mathematics
Advanced Techniques In Mass Spectrometry For Qualitative And Quantitative Protein Characterization, Andrew Boissy Dykstra
Advanced Techniques In Mass Spectrometry For Qualitative And Quantitative Protein Characterization, Andrew Boissy Dykstra
Doctoral Dissertations
Though mass spectrometry has earned a central role in the field of proteomics due to its versatility in a wide range of experiments, challenges and complications are still encountered when using mass spectrometry to characterize protein structures, post-translational modifications (PTMs), and abundances. In this dissertation, analytical methods utilizing mass spectrometry have been developed to address challenges associated with both qualitative and quantitative protein characterization. The effectiveness of using multiple pepsin-like proteases, both separately and in mixtures, combined with online proteolysis using a special triaxial probe has been demonstrated on an amyloid beta peptide related to the onset of Alzheimer’s disease. …
Spatially Resolved Laser And Thermal Desorption/Ionization Coupled With Mass Spectrometry, Olga Sergeevna Ovchinnikova
Spatially Resolved Laser And Thermal Desorption/Ionization Coupled With Mass Spectrometry, Olga Sergeevna Ovchinnikova
Doctoral Dissertations
The work discussed in this dissertation is aimed at creating novel approaches to chemical imaging that ultimately allow for submicron resolution. This goal has been approached from two direction using laser based desorption and coupling it with an AFM using apertureless tip-enhanced laser ablation/ionization. The second direction was through the development a new approach to thermal desorption based mass spectrometry experiments by using a proximal probe to spatially desorb the surface and ionizing the plume of neutrals using a secondary ionization source at atmospheric pressure. The thermal desorption approach allows for the easy scaling of the technique all the way …