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Full-Text Articles in Physical Sciences and Mathematics
Total Internal Reflection: Applications In Nonlinear Microscopy And Fluorescence Anisotropy, Brandon Colon
Total Internal Reflection: Applications In Nonlinear Microscopy And Fluorescence Anisotropy, Brandon Colon
Doctoral Dissertations
As technology advances to harness new energies and to create new cures, the sophistication of analysis grows not only in depth but in efficiency. Total internal reflection (TIR) has been coupled to microscopy leveraging its unique optical phenomenon on a breadth of topics. In this dissertation, the work presented will show how TIR was applied in two different instrumental analyses to evaluate two unique and complex systems. The first project features TIR paired with the transient absorption microscopy (TAM), a nonlinear optical technique, to gauge solvent mixing and diffusion in microreactors. Microreactors gained acclaim for their ability to produce high …
Instrument Development For High Sensitivity Size Characterization Of Lipid Vesicles And Other Biological Macromolecules Via Taylor Dispersion Analysis, Meagan Moser
Doctoral Dissertations
Just as humans communicate with other humans, the cells in our bodies communicate with each other through various, often complex, mechanisms. Cell-to-cell transmission of small molecules, lipids, proteins, peptides, or nucleic acids can be mediated by extracellular lipid vesicles called exosomes. Exosomes have been found to play a role in the delivery of regulatory molecules from one cell to another, serving as a universal communication mechanism. Currently, there is an emerging focus on characterizing exosome communication dynamics. Understanding exosome mechanisms of cell-to-cell communication requires accurate measurements of the spatiotemporal and chemical dynamics of exosome secretion. No current analytical approach offers …