Physical Sciences and Mathematics Commons^™

The Low Resolution Structure Of Apoa1 In Spherical High Density Lipoprotein Revealed By Small Angle Neutron Scattering, Zhiping Wu, Valentin Gogonea, Xavier Lee, Roland P. May, Vitaliy Pipich, Matthew A. Wagner, Arundhati Undurti, Thomas C. Tallant, Camelia Baleanu Gogonea, Francesca Charlton, Alexander I. Ioffe, Joseph A. Didonato, Kerry Anne Rye, Stanley L. Hazen

Chemistry Faculty Publications

Spherical high density lipoprotein (sHDL), a key player in reverse cholesterol transport and the most abundant form of HDL, is associated with cardiovascular diseases. Small angle neutron scattering with contrast variation was used to determine the solution structure of protein and lipid components of reconstituted sHDL. Apolipoprotein A1, the major protein of sHDL, forms a hollow structure that cradles a central compact lipid core. Three apoA1 chains are arranged within the low resolution structure of the protein component as one of three possible global architectures: (i) a helical dimer with a hairpin (HdHp), (ii) three hairpins (3Hp), or (iii) an …