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Role Of The 3'Lghrr In Tcdd-Induced Suppression Of The Immunoglobulin Heavy Chain, Jayharsh L. Panchal, Eric J. Romer, David Ellis, Tharu Fernando, Courtney E. W. Sulentic

Symposium of Student Research, Scholarship, and Creative Activities Materials

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) inhibits antibody secretion and immunoglobulin heavy chain (IgH) expression. Our previous work has shown that a possible mechanism for inhibition of IgH expression could be inhibition of the 3’IgH regulatory region (3’IgHRR). The 3’IgHRR has four enhancer regions (hs3a; hs1,2; hs3b; hs4) which are purported to control IgH gene expression. Previously we demonstrated a sensitive inhibition by TCDD of LPS-induced 3’IgHRR activity which is correlated with an inhibition of LPS-induced hs1,2 enhancer activity; however, the hs4 enhancer showed TCDD-induced activation following the co-treatment of TCDD and LPS. Therefore, the objective of the current study was to determine if …