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Open Access. Powered by Scholars. Published by Universities.®

Western Washington University

2019

Sortase A

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Full-Text Articles in Physical Sciences and Mathematics

Engineering Sortase; Activity And Selectivity Of New Hybrid And Ancestral Variants Of Sortase A, Sarah Struyvenberg May 2019

Engineering Sortase; Activity And Selectivity Of New Hybrid And Ancestral Variants Of Sortase A, Sarah Struyvenberg

Scholars Week

Bacterial sortase enzymes are a beneficial tool in innovative mechanisms of protein engineering. However, important limitations to utilization of sortases for engineered purposes exist; namely, that sortase A (SrtA) is a relatively poor enzyme and very specific for the substrate containing LPATXG motif. Exciting previous work from our collaborators reveals that sortases from different species recognize different sequences and that activity can vary. Therefore, we wanted to create and investigate hybrid sortase enzymes between SrtA from S. aureus and Streptococcus pneumoniae, wherein we swapped a substrate-interacting loop between the beta-E and beta-F strands. Our hypothesis is that these residues are …

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Isopeptide Ligations Catalyzed By Streptococcus Suis Sortase A, Sarah Bowersox Jan 2019

Isopeptide Ligations Catalyzed By Streptococcus Suis Sortase A, Sarah Bowersox

WWU Graduate School Collection

Chemically modified proteins are critical components of modern therapeutics and basic research. To generate non-natural protein derivatives, bacterial sortase enzymes have been effective due to their ability to catalyze selective ligations between protein targets and functional groups that are uncommon in nature. Thus far, the enzymatic approach using sortase has been limited to modifications at the termini of peptide chains. Here we describe efforts to develop a sortase-mediated strategy for the formation of isopeptide bonds at the side chains of internal lysine residues. To this end, we have identified a sortase A homolog from Streptococcus suis (SrtAsuis) that …

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