Physical Sciences and Mathematics Commons^™

Structural Studies On The Mechanism Of Argyrin B And The L12 – L11 Ribosomal Protein Interface, Christopher Swanson

WWU Graduate School Collection

The mechanism of action of argyrin B and the molecular interactions of L11 and L12 has been undetermined and underrepresented in the research of bacterial translation. This work seeks to examine the mechanism of action of argyrin B by performing in vitro structural studies of its interaction with its specific target protein elongation factor-G. We demonstrate that argyrin B inhibits translation, and allows GTPase activity to proceed, contrary to assumptions made in prior research. We determine that ribosome recycling is the likely step through which argyrin B acts as an antibiotic, since translocation is unaffected by argyrin B and association …