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Full-Text Articles in Physical Sciences and Mathematics
Genetic Analysis Of Acetone Carboxylase In Azotobacter Vinelandii, Jessica Gardner
Genetic Analysis Of Acetone Carboxylase In Azotobacter Vinelandii, Jessica Gardner
Undergraduate Honors Capstone Projects
Acetone carboxylase, an inducible multimeric enzyme found in several bacteria, catalyzes the carboxylation of acetone to produce acetoacetate. This is an important reaction for the bacteria because, upon addition of coenzyme A, the acetoacetate can be converted to acetyl-CoA, a common metabolic intermediate. Azotobacter vinelandii, one of the bacteria that produces acetone carboxylase, has two different genes encoding this enzyme. We knocked out the first of these by disrupting it with a kanamycin cassette to help us understand the function of the second gene. Knocking out the gene will also make possible the use of site-directed mutagenesis to further study …
Site-Directed Mutagenesis Of The Nitrogenase Fe-Protein From Azotobacter Vinelandii, Talmage L. Shill
Site-Directed Mutagenesis Of The Nitrogenase Fe-Protein From Azotobacter Vinelandii, Talmage L. Shill
Undergraduate Honors Capstone Projects
Nitrogen fixation is the chemical reaction of dinitrogen (N2) being reduced to ammonia (NH3). This reaction occurs naturally by nitrogen fixing microorganisms which contain the enzyme nitrogenase. The overall reaction is written below.