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Full-Text Articles in Physical Sciences and Mathematics
Malonate-Bound Structure Of The Glycerophosphodiesterase From Enterobacter Aerogenes (Gpdq) And Characterization Of The Native Fe2+ Metal-Ion Preference, Colin J. Jackson, Kieran S. Hadler, Paul D. Carr, Aaron J. Oakley, Sylvia Yip, Gerhard Schenk, David L. Ollis
Malonate-Bound Structure Of The Glycerophosphodiesterase From Enterobacter Aerogenes (Gpdq) And Characterization Of The Native Fe2+ Metal-Ion Preference, Colin J. Jackson, Kieran S. Hadler, Paul D. Carr, Aaron J. Oakley, Sylvia Yip, Gerhard Schenk, David L. Ollis
Faculty of Science - Papers (Archive)
The structure of a malonate-bound form of the glycerophosphodiesterase from Enterobacter aerogenes, GpdQ, has been refined at a resolution of 2.2 A ̊ to a final R factor of 17.1%. The structure was originally solved to 2.9 A ̊ resolution using SAD phases from Zn2+ metal ions introduced into the active site of the apoenzyme [Jackson et al. (2007), J. Mol. Biol. 367, 1047–1062]. However, the 2.9 A ̊ resolution was insufficient to discern significant details of the architecture of the binuclear metal centre that constitutes the active site. Furthermore, kinetic analysis revealed that the enzyme lost a significant amount …
Reactions Of The Hydroperoxide Anion With Dimethyl Methylphosphonate In An Ion Trap Mass Spectrometer: Evidence For A Gas Phase A-Effect, Andrew M Mcanoy, Martin Paine, Stephen J. Blanksby
Reactions Of The Hydroperoxide Anion With Dimethyl Methylphosphonate In An Ion Trap Mass Spectrometer: Evidence For A Gas Phase A-Effect, Andrew M Mcanoy, Martin Paine, Stephen J. Blanksby
Faculty of Science - Papers (Archive)
The gas phase degradation reactions of the chemical warfare agent (CWA) simulant, dimethyl methylphosphonate (DMMP), with the hydroperoxide anion (HOO–) were investigated using a modified quadrupole ion trap mass spectrometer. The HOO– anion reacts readily with neutral DMMP forming two significant product ions at m/z 109 and m/z 123. The major reaction pathways correspond to (i) the nucleophilic substitution at carbon to form [CH3P(O)(OCH3)O]– (m/z 109) in a highly exothermic process and (ii) exothermic proton transfer. The branching ratios of the two reaction pathways, 89% and 11% respectively, indicate that the …