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Full-Text Articles in Physical Sciences and Mathematics
Affinity Chromatography: A Historical Perspective, David S. Hage, Ryan E. Matsuda
Affinity Chromatography: A Historical Perspective, David S. Hage, Ryan E. Matsuda
David Hage Publications
Affinity chromatography is one of the most selective and versatile forms of liquid chromatography for the separation or analysis of chemicals in complex mixtures. This method makes use of a biologically related agent as the stationary phase, which provides an affinity column with the ability to bind selectively and reversibly to a given target in a sample. This review examines the early work in this method and various developments that have lead to the current status of this technique. The general principles of affinity chromatography are briefly described as part of this discussion. Past and recent efforts in the generation …
Affinity Monolith Chromatography: A Review Of Principles And Recent Analytical Applications, Erika L. Pfaunmiller, Marie Laura Paulemond, Courtney M. Dupper, David S. Hage
Affinity Monolith Chromatography: A Review Of Principles And Recent Analytical Applications, Erika L. Pfaunmiller, Marie Laura Paulemond, Courtney M. Dupper, David S. Hage
David Hage Publications
Affinity monolith chromatography (AMC) is a type of liquid chromatography that uses a monolithic support and a biologically-related binding agent as a stationary phase. AMC is a powerful method for the selective separation, analysis or studies of specific target compounds in a sample. This review discusses the basic principles of AMC and recent developments or applications of this method, with particular emphasis being given to work that has appeared in the last five years. Various materials that have been used to prepare columns for AMC are examined, including organic monoliths, silica monoliths, agarose monoliths and cryogels. These supports have been …