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Full-Text Articles in Physical Sciences and Mathematics
Clinical And Pharmaceutical Applications Of Affinity Ligands In Capillary Electrophoresis: A Review, Chenhua Zhang, Ashley G. Woolfork, Kyungah Suh, Susan Ovbude, Cong Bi, Marawan Elzoeiry, David S. Hage
Clinical And Pharmaceutical Applications Of Affinity Ligands In Capillary Electrophoresis: A Review, Chenhua Zhang, Ashley G. Woolfork, Kyungah Suh, Susan Ovbude, Cong Bi, Marawan Elzoeiry, David S. Hage
David Hage Publications
Affinity capillary electrophoresis (ACE) is a separation technique that combines a biologically-related binding agent with the separating power and efficiency of capillary electrophoresis. This review will examine several classes of binding agents that have been used in ACE and applications that have been described for the resulting methods in clinical or pharmaceutical analysis. Binding agents that will be considered are antibodies, aptamers, lectins, serum proteins, carbohydrates, and enzymes. This review will also describe the various formats in which each type of binding agent has been used in CE, including both homogeneous and heterogeneous methods. Specific areas of applications that will …
Studies By Biointeraction Chromatography Of Binding By Phenytoin Metabolites To Human Serum Albumin, Corey M. Ohnmacht, Shirley Chen, Zenghan Tong, David S. Hage
Studies By Biointeraction Chromatography Of Binding By Phenytoin Metabolites To Human Serum Albumin, Corey M. Ohnmacht, Shirley Chen, Zenghan Tong, David S. Hage
David Hage Publications
Biointeraction studies based on high performance affinity chromatography were used to investigate the binding of human serum albumin (HSA) to two major phenytoin metabolites: 5-(3-hydroxyphenyl)-5-phenylhydantoin (m-HPPH) and 5-(4-hydroxyphenyl)-5-phenylhydantoin (p-HPPH). This was initially examined by conducting self-competition zonal elution experiments in which m-HPPH or p-HPPH were placed in both the mobile phase and injected sample. It was found that each metabolite had a single major binding site on HSA. Competitive zonal elution experiments using l-tryptophan, warfarin, digitoxin, and cis-clomiphene as site-selective probes indicated that m-HPPH and p-HPPH were interacting with the indolebenzodiazepine …