Physical Sciences and Mathematics Commons^™

Chromatographic Analysis Of Carbamazepine Binding To Human Serum Albumin: Ii. Comparison Of The Schiff Base And N-Hydroxysuccinimide Immobilization Methods, Hee Seung Kim, Rangan Mallik, David S. Hage

David Hage Publications

Recent studies with carbamazepine on human serum albumin (HSA) columns have noted an appreciable degree of non-specific binding on supports prepared by the Schiff base immobilization method. This work examines an alternative immobilization method for HSA based on N-hydroxysuccinimide (NHS)-activated silica. This support was prepared by reacting HPLC-grade silica directly with disuccinimidyl carbonate. The resulting material was compared to an HSA support prepared by the Schiff base method in terms of its activity for carbamazepine and non-specific interactions with this drug. When examined by frontal analysis, both supports gave comparable association equilibrium constants for carbamazepine interactions with HSA ((0.53–0.55) …