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Full-Text Articles in Physical Sciences and Mathematics

Chromatographic Immunoassays: Strategies And Recent Developments In The Analysis Of Drugs And Biological Agents, Ryan E. Matsuda, Elliott Rodriguez, Doddavenkatanna Suresh, David S. Hage Jan 2015

Chromatographic Immunoassays: Strategies And Recent Developments In The Analysis Of Drugs And Biological Agents, Ryan E. Matsuda, Elliott Rodriguez, Doddavenkatanna Suresh, David S. Hage

David Hage Publications

A chromatographic immunoassay is a technique in which an antibody or antibodyrelated agent is used as part of a chromatographic system for the isolation or measurement of a specific target. Various binding agents, detection methods, supports and assay formats have been developed for this group of methods, and applications have been reported that range from drugs, hormones and herbicides to peptides, proteins and bacteria. This review discusses the general principles and applications of chromatographic immunoassays, with an emphasis being given to methods and formats that have been developed for the analysis of drugs and biological agents. The relative advantages or …


Affinity Chromatography: A Historical Perspective, David S. Hage, Ryan E. Matsuda Jan 2015

Affinity Chromatography: A Historical Perspective, David S. Hage, Ryan E. Matsuda

David Hage Publications

Affinity chromatography is one of the most selective and versatile forms of liquid chromatography for the separation or analysis of chemicals in complex mixtures. This method makes use of a biologically related agent as the stationary phase, which provides an affinity column with the ability to bind selectively and reversibly to a given target in a sample. This review examines the early work in this method and various developments that have lead to the current status of this technique. The general principles of affinity chromatography are briefly described as part of this discussion. Past and recent efforts in the generation …