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Full-Text Articles in Physical Sciences and Mathematics
Determination Of Conditions For Oye In Which There Is No Ion Present In The Active Site, Melissa J. Morris
Determination Of Conditions For Oye In Which There Is No Ion Present In The Active Site, Melissa J. Morris
Honors Theses
Old Yellow Enzyme (OYE) has been the subject of extensive research since the early 1930's. However, the structure of OYE was not determined until 1994, and its physiological function still remains a mystery. Investigations into the function of OYE involve uncovering the details of protein-substrate interactions through the use of x-ray crystallography. OYE is known to oxidize NADPH, but attempts to crystallize the OYE-NADPH bound complex have been hampered by the presence of chloride in the active site of OYE. This competition prevents an accurate assessment of the structure of OYE bound to NADPH. This study will examine the possibility …
Inclusion Processes Of Cyclodextrins And Their Polymers: A Molecular Modeling Study, Stacey L. Fellows
Inclusion Processes Of Cyclodextrins And Their Polymers: A Molecular Modeling Study, Stacey L. Fellows
Honors Theses
Cyclodextrins (COs) and cyclodextrin polymers (CDPs) are molecules capable ot creating host/guest complexes, where a host molecule allows another molecule, a guest, to bind to it without either molecule losing its molecular identity. CDs are hydrophilic t,, 11, contain a unique hydrophobic cavity that can attract organic guest molecules. CDP is a derivative of CD and consist of two or more CD units connected by chains of glyceryl linkages (-CHrCHOH-CH2-O-). CDPs are typically more hydrophilic- than the CD monomers making them more useful for applications. However, behavior of the CDP is not as well-cinderstood as that of the monomer. Computer …
Design And Application Of A Compact, Multipurpose, Laser-Based Spectrometer, Andrew M. Leach
Design And Application Of A Compact, Multipurpose, Laser-Based Spectrometer, Andrew M. Leach
Honors Theses
A compact laser-based spectrometer has been designed and characterized. This design of this instrument gives it the ability to collect absorbance, fluorescence, and scattering signals simultaneously. A diode laser is used as the excitation for this instrument. Several optical arrangements have been tested in order to allow for the best possible signal collection. Electronics have been designed and constructed in order to facilitate the simultaneous collection of signals. This instrument has been characterized using several dyes and scattering agents. Fluorescence limits of detection for Methylene Blue and Nile Blue A have been calculated at 2 ppb. The spectrometer has been …
Flow Injection Analysis With A Laser-Based Detector For On-Line Determination Of Sulfide, Glucose, And Ethanol, Manisha Tinani
Flow Injection Analysis With A Laser-Based Detector For On-Line Determination Of Sulfide, Glucose, And Ethanol, Manisha Tinani
Honors Theses
In this research a flow injection (FI) apparatus with a laser-based detector is characterized using three chemical systems and the results are compared to those obtained by other researchers using conventional instrumentation. Each chemical system involves Methylene Blue (MR), a common redox indicator. A 670-nm diode laser is used as the excitation source, and the resulting fluorescence is measured. In the first system, MB is formed as a product of the reaction of N,N-dimethylphenylenediamine (DMPD), iron (lll) and sulfide in the presence of acid. A three-line FI manifold is employed. The amount of sulfide in water samples, including a complicated …
The Crystallization Of Estrogen-Binding Protein, Jeffrey R. Weinstein
The Crystallization Of Estrogen-Binding Protein, Jeffrey R. Weinstein
Honors Theses
Candida albicans is a yeast that is known to target humans with weakened immune systems. The growth rate and invasiveness of C. albicans is stimulated in the presence of estrogens. Experimental evidence suggests that the estrogen-binding protein (EBP) from C. albicans may be involved in the estrogen-induced increase in growth rate. In order to investigate the possible involvement of EBP in this process, work is underway to determine the atomic structures of EBP alone and bound to estrogen. Protein crystals of EBP have been grown at 4°C in the presence and absence of estrogen using vapor diffusion techniques from a …
Synthesis And Characterization Of Β-Cyclodextrin Polymers, Jodie L. Iannacone
Synthesis And Characterization Of Β-Cyclodextrin Polymers, Jodie L. Iannacone
Honors Theses
Cyclodextrin polymers (CDPs) are water Joluble polymers composed of either a, fl, or y cyclodextrin (CDl monomers. TI,ese commercially available polymers are synthesized ming epichlorohydrin and consist of CD monomers joined by repeating glycer;l linkers (-(CH2-CHOH-CH2-ln) with an average n value of 12-15. GPC analysis of these polymers indicate two major component peaks that have molecular weights (MW) of 2,000 (one CD/ polymer chain) and 9-10,000 (4-5 CDs/polymer chain). These polymers have been used to study the binding interactions of various fluorescence probes. It has been shown that the pyrene fluorescence lifetime increases and its emission I/III ratio decreases in …
Introduction Of The Gas Chromatograph Into The Introductory Chemistry Laboratory, Jonathan Calvert Wedvik
Introduction Of The Gas Chromatograph Into The Introductory Chemistry Laboratory, Jonathan Calvert Wedvik
Honors Theses
No abstract provided.
Flash Photochemical Kinetics Of Chromium Carbonyls, Jason David Katz
Flash Photochemical Kinetics Of Chromium Carbonyls, Jason David Katz
Honors Theses
Chromium hexacarbonyl, Cr(C0)6, has been shown to be a very effective photochemical catalyst for many reactions, such as the 1,4- hydrogenation and 1,4-hydrosilatlon of conjugated 1,3-dienes resulting in the formation of a cis alkene product in greater than a 99% percent yield. The catalytic power of Cr(CO)6 has merited its study by many investigators, mostly with regard to the structure of the catalytic intermediates, rather than the actual kinetics of individual steps in the mechanism. The goal of this study is to construct a database of both rate and mechanistic information on the reactions of Cr(CO)6 with various alkenes and …