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Full-Text Articles in Physical Sciences and Mathematics
Ultra-Filtration Of Human Serum For Improved Quantitative Analysis Of Low Molecular Weight Biomarkers Using Atr-Ir Spectroscopy, Franck Bonnier, Helene Blasco, Clement Wasselet, Guillaume Brachet, Renaud Respaud, Luis Felipe Cs. Carvalho, Dominque Bertrand, Mathew J. Baker, Hugh Byrne, Igor Chourpa
Ultra-Filtration Of Human Serum For Improved Quantitative Analysis Of Low Molecular Weight Biomarkers Using Atr-Ir Spectroscopy, Franck Bonnier, Helene Blasco, Clement Wasselet, Guillaume Brachet, Renaud Respaud, Luis Felipe Cs. Carvalho, Dominque Bertrand, Mathew J. Baker, Hugh Byrne, Igor Chourpa
Articles
Infrared spectroscopy is a reliable, rapid and cost effective characterisation technique, delivering a molecular finger print of the sample. It is expected that its sensitivity would enable detection of small chemical variations in biological samples associated with disease. ATR-IR is particularly suitable for liquid sample analysis and, although air drying is commonly performed before data collection, just a drop of human serum is enough for screening and early diagnosis. However, the dynamic range of constituent biochemical concentrations in the serum composition remains a limiting factor to the reliability of the technique. Using glucose as a model spike in human serum, …
Screening The Low Molecular Weight Fraction Of Human Serum Using Atr-Ir Spectroscopy, Franck Bonnier, Matthew J. Baker, Hugh Byrne
Screening The Low Molecular Weight Fraction Of Human Serum Using Atr-Ir Spectroscopy, Franck Bonnier, Matthew J. Baker, Hugh Byrne
Articles
Vibrational spectroscopic techniques can detect small variations in molecular content, linked with disease, showing promise for screening and early diagnosis. Biological fluids, particularly blood serum, are potentially valuable for diagnosis purposes. The so-called Low Molecular Weight Fraction (LMWF) contains the associated peptidome and metabolome and has been identified as potentially the most relevant molecular population for disease-associated biomarker research. Although vibrational spectroscopy can deliver a specific chemical fingerprint of the samples, the High Molecular Weight Fraction (HMWF), composed of the most abundant serum proteins, strongly dominates the response and ultimately makes the detection of minor spectral variations a challenging task. …