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Full-Text Articles in Physical Sciences and Mathematics
Establishment Of Photo-Activated Localization Microscopy (Palm) For Imaging Signaling Complexes On The Surfaces Of Cells, Bradley E. Iverson
Establishment Of Photo-Activated Localization Microscopy (Palm) For Imaging Signaling Complexes On The Surfaces Of Cells, Bradley E. Iverson
The Journal of Undergraduate Research
Photoactivated Localization Microscopy (PALM) permits visualization of single molecules inside cells at ~10 times the resolution of existing optical microscopes. The 10-50 nanometer resolution afforded by PALM is ideally suited to studying the organization of receptor signal transduction occurring on the surfaces of cells. Initial efforts presented here focused on cells expressing Fc receptor fused to photoactivatable green fluorescent protein (paGFP). A small fraction of the paGFP molecules were photoactivated using a 405 nm laser pulse, resulting in single molecule spots that could then be imaged using 488 nm laser excitation until they photobleached. Both lasers illuminated the sample through …
The Journal Of Undergraduate Research: Volume 10
The Journal Of Undergraduate Research: Volume 10
The Journal of Undergraduate Research
This is the complete issue of the South Dakota State University Journal of Undergraduate Research, Volume 10.