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Full-Text Articles in Physical Sciences and Mathematics

The Contribution Of Fe (Iii) And Humic Acid Reduction To Ecosystem Respiration In Drained Thaw Lake Basins Of The Arctic Coastal Plain, David A. Lipson, Ted K. Raab, Dominic Goria, Jaime Zlamal May 2013

The Contribution Of Fe (Iii) And Humic Acid Reduction To Ecosystem Respiration In Drained Thaw Lake Basins Of The Arctic Coastal Plain, David A. Lipson, Ted K. Raab, Dominic Goria, Jaime Zlamal

Ted K. Raab

Previous research showed that anaerobic respiration using iron (Fe) oxides as terminal electron acceptor contributed substantially to ecosystem respiration (ER) in a drained thaw lake basin (DTLB) on the Arctic coastal plain. As DTLB age, the surface organic layer thickens, progressively burying the Fe-rich mineral layers. We therefore hypothesized that Fe (III) availability and Fe reduction would decline with basin age. We studied four DTLB across an age gradient, comparing seasonal changes in the oxidation state of dissolved and extractable Fe pools and the estimated contribution of Fe reduction to ER. The organic layer thickness did not strictly increase with …


Evidence That Glucose Is The Major Transferred Metabolite In Dinoflagellate–Cnidarian Symbiosis, Matthew S. Burriesci, Ted K. Raab, John R. Pringle Oct 2012

Evidence That Glucose Is The Major Transferred Metabolite In Dinoflagellate–Cnidarian Symbiosis, Matthew S. Burriesci, Ted K. Raab, John R. Pringle

Ted K. Raab

Reef-building corals and many other cnidarians are symbiotic with dinoflagellates of the genus Symbiodinium. It has long been known that the endosymbiotic algae transfer much of their photosynthetically fixed carbon to the host and that this can provide much of the hostʼs total energy. However, it has remained unclear which metabolite(s) are directly translocated from the algae into the host tissue. We reexamined this question in the small sea anemone Aiptasia using labeling of intact animals in the light with 13C-bicarbonate, rapid homogenization and separation of animal and algal fractions, and analysis of metabolite labeling by gas chromatography-mass spectrometry. We …


Water Table Height And Microtopography Control Biogeochemical Cycling In An Arctic Coastal Tundra Ecosystem, David A. Lipson, Donatella Zona, Ted K. Raab, Francis Bozzolo, Marguerite Mauritz, Walter C. Oechel Jan 2012

Water Table Height And Microtopography Control Biogeochemical Cycling In An Arctic Coastal Tundra Ecosystem, David A. Lipson, Donatella Zona, Ted K. Raab, Francis Bozzolo, Marguerite Mauritz, Walter C. Oechel

Ted K. Raab

Drained thaw lake basins (DTLB’s) are the dominant land form of the Arctic Coastal Plain in northern Alaska. The presence of continuous permafrost prevents drainage and so water tables generally remain close to the soil surface, creating saturated, suboxic soil conditions. However, ice wedge polygons produce microtopographic variation in these landscapes, with raised areas such as polygon rims creating more oxic microenvironments. The peat soils in this ecosystem store large amounts of organic carbon which is vulnerable to loss as arctic regions continue to rapidly warm, and so there is great motivation to understand the controls over microbial activity in …


Reduction Of Iron (Iii) And Humic Substances Plays A Major Role In Anaerobic Respiration In An Arctic Peat Soil, David A. Lipson, Mony Jha, Ted K. Raab, Walter C. Oechel Dec 2010

Reduction Of Iron (Iii) And Humic Substances Plays A Major Role In Anaerobic Respiration In An Arctic Peat Soil, David A. Lipson, Mony Jha, Ted K. Raab, Walter C. Oechel

Ted K. Raab

Arctic peat soils contain vast reserves of organic C and are largely anaerobic. However, anaerobic respiration, particularly the role of Fe(III) and humic substances as electron acceptors, is not well understood in such ecosystems. We investigated these processes in a drained thaw lake basin on the Arctic coastal plain near Barrow, Alaska. We measured concentrations of soluble Fe and other potential electron acceptors, described the microbial community, and performed experiments in the laboratory and field to measure net rates of Fe(III) reduction and the relationship of this process to C cycling. In most areas within the basin, aerobic conditions existed …


The Coconut Palm, Cocos Nucifera, Impacts Forest Composition And Soil Characteristics At Palmyra Atoll, Central Pacific, Hillary S. Young, Ted K. Raab, Douglas J. Mccauley, Amy A. Briggs, Rodolfo Dirzo Jan 2010

The Coconut Palm, Cocos Nucifera, Impacts Forest Composition And Soil Characteristics At Palmyra Atoll, Central Pacific, Hillary S. Young, Ted K. Raab, Douglas J. Mccauley, Amy A. Briggs, Rodolfo Dirzo

Ted K. Raab

Cocos nucifera, the coconut palm, has a pantropical distribution and reaches near monodominance in many atolls, low lying islands and coastal regions. This paper examines the ecological correlation between C. nucifera abundance and changes in forest structure, floristic diversity and forest soil characteristics. Cumulatively, these data show that C. nucifera has important impacts on floristic, structural and soil characteristics of forests where it becomes dominant. Given the high proportion of tropical coastal areas in which C. nucifera is now naturalized and abundant, this likely has important implications for coastal forest diversity and structure.


The Rhizosphere: A Synchrotron- Based View Of Nutrient Flow In The Root Zone, Theodore K. Raab, David A. Lipson Jan 2010

The Rhizosphere: A Synchrotron- Based View Of Nutrient Flow In The Root Zone, Theodore K. Raab, David A. Lipson

Ted K. Raab

At two energy “endpoints” of the EM spectrum accessible to synchrotron biologists (IR radiation and soft X-rays), we have found experimental methods providing unique opportunities to observe processes and test hypotheses essential to understanding plantsoil- microbe interactions. Availability of IR synchrotron beamlines will continue to expand worldwide, and we hope that plant and microbial biologists will incorporate the type of spectral data presented in this chapter as just one of a legion of “-omics” methods. Considering the shear flux of C through belowground ecosystems annually (and how poorly constrained they are in many regions), synchrotrons must continue to demand the …


Ecological And Agricultural Applications Of Synchrotron Ir Microscopy, Ted K. Raab, John P. Vogel Sep 2004

Ecological And Agricultural Applications Of Synchrotron Ir Microscopy, Ted K. Raab, John P. Vogel

Ted K. Raab

The diffraction-limited spot size of synchrotron-based IR microscopes provides cell-specific, spectrochemical imaging of cleared leaf, stem and root tissues of the model genetic organism Arabidopsis thaliana, and mutant plants created either by T-DNA insertional inactivation or chemical mutagenesis. Spectra in the wavelength region from 6 to 12 microns provide chemical and physical information on the cell wall polysaccharides of mutants lacking particular biosynthetic enzymes (‘‘Cellulose synthase-like’’ genes). In parallel experiments, synchrotron IR microscopy delineates the role of Arabidopsis cell wall enzymes as susceptibility factors to the fungus Erysiphe cichoracearum, a causative agent of powdery mildew disease. Three genes, pmr4, pmr5, …


Pmr6, A Pectate Lyase–Like Gene Required For Powdery Mildew Susceptibility In Arabidopsis, John P. Vogel, Ted K. Raab, Celine Schiff, Shauna C. Somerville Sep 2002

Pmr6, A Pectate Lyase–Like Gene Required For Powdery Mildew Susceptibility In Arabidopsis, John P. Vogel, Ted K. Raab, Celine Schiff, Shauna C. Somerville

Ted K. Raab

The plant genes required for the growth and reproduction of plant pathogens are largely unknown. In an effort to identify these genes, we isolated Arabidopsis mutants that do not support the normal growth of the powdery mildew pathogen Erysiphe cichoracearum. Here, we report on the cloning and characterization of one of these genes, PMR6. PMR6 encodes a pectate lyase-like protein with a novel C-terminal domain. Consistent with its predicted gene function, mutations in PMR6 alter the composition of the plant cell wall, as shown by Fourier transform infrared spectroscopy. pmr6-mediated resistance requires neither salicylic acid nor the ability to perceive …


Visualizing Rhizosphere Chemistry Of Legumes With Mid-Infrared Synchrotron Radiation, Ted K. Raab, Michael C. Martin May 2001

Visualizing Rhizosphere Chemistry Of Legumes With Mid-Infrared Synchrotron Radiation, Ted K. Raab, Michael C. Martin

Ted K. Raab

A bright synchrotron light source operated by the Lawrence Berkeley National Laboratory served as an external source for infrared (IR) microscopy of plant root microcosms. Mid-IR light from synchrotrons is 2-3 orders of magnitude brighter than conventional sources, providing contrast based on the chemical information in the reflected signal at a spatial resolution near the diffraction-limit of 3-10 microns. In an experiment using plant root microcosms fitted with zinc selenide IR-transmissive windows (50 mm x 20 mm x 1 mm), we describe chemical differences and similarities within the root zone of mung bean (Vigna radiata L.), grown with or without …


Soil Amino Acid Utilization Among Species Of The Cyperaceae: Plant And Soil Processes, Ted K. Raab, David A. Lipson, Steven K. Scmidt, Russ K. Monson Jan 1999

Soil Amino Acid Utilization Among Species Of The Cyperaceae: Plant And Soil Processes, Ted K. Raab, David A. Lipson, Steven K. Scmidt, Russ K. Monson

Ted K. Raab

Amino acids are released during the decomposition of soil organic matter and have been shown to be utilized as a nitrogen source by some non-mycorrhizal species in the family Cyperaceae (the sedge family). Twelve out of 13 Cyperaceae species examined in the current study were capable of absorbing soil amino acids in the non-mycorrhizal state. With two exceptions (two species in the genus Kobresia), species from subalpine or alpine habitats exhibited lower rates of total nitrogen uptake compared to species from more temperate habitats, which is possibly explained by lower growth rates in the alpine and subalpine species and a …