Physical Sciences and Mathematics Commons^™

Can Acute Dermal Systemic Toxicity Tests Be Replaced With Oral Tests? A Comparison Of Route-Specific Systemic Toxicity And Hazard Classifications Under The Globally Harmonized System Of Classification And Labelling Of Chemicals (Ghs), Nigel P. Moore, David J. Andrew, Donald L. Bjerke, Stuart Creton, David Dreher, Thomas Holmes, Pilar Prieto, Troy Seidle, Tim G. Rowan

Troy Seidle, PhD

Acute systemic toxicity data (LD50 values) and hazard classifications derived in the rat following oral administration and dermal application have been analysed to examine whether or not orally-derived hazard classification or LD50 values can be used to determine dermal hazard classification. Comparing the oral and dermal classifications for 335 substances derived from oral and dermal LD50 values respectively revealed 17% concordance, and indicated that 7% of substances would be classified less severely while 76% would be classified more severely if oral classifications were applied directly to the dermal route. In contrast, applying the oral LD50 values within the dermal classification …

An Evaluation Of The Us High Production Volume (Hpv) Chemical-Testing Programme: A Study In (Ir)Relevance, Redundancy And Retro Thinking, Andrew Nicholson, Jessica Sandler, Troy Seidle

Troy Seidle, PhD

Under the US Environmental Protection Agency (EPA) High Production Volume (HPV) Challenge Programme, chemical companies have volunteered to conduct screening-level toxicity tests on approximately 2800 widely-used industrial chemicals. Participating companies are committed to providing available toxicity information to the EPA and presenting testing proposals for review by the EPA and posting on the EPA Web site as public information. People for the Ethical Treatment of Animals (PETA) and a coalition of animal protection organisations have reviewed all the test plans submitted by the participating chemical companies for compliance with the original HPV framework, as well as with animal welfare guidelines …

The Development Of New Concepts For Assessing Reproductive Toxicity Applicable To Large Scale Toxicological Programmes, S. Bremer, C. Pellizzer, S. Hoffmann, T. Seidle, T. Hartung

Troy Seidle, PhD

Large scale toxicological testing programmes which are currently ongoing such as the new European chemical legislation REACH require the development of new integrated testing strategies rather than applying traditional testing schemes to thousands of chemicals. The current practice of requiring in vivo testing for every possible adverse effect endanger the success of these programmes due (i) to limited testing facilities and sufficient capacity of scientific/technical knowledge for reproductive toxicity; (ii) an unacceptable number of laboratory animals involved (iii) an intolerable number of chemicals classified as false positive.

A key aspect of the implementation of new testing strategies is the determination …

A Modular One-Generation Reproduction Study As A Flexible Testing System For Regulatory Safety Assessment, Richard Vogel, Troy Seidle, Horst Spielmann

Troy Seidle, PhD

The European Union’s Registration, Evaluation and Authorisation of Chemicals (REACH) legislation mandates testing and evaluation of approximately 30,000 existing substances within a short period of time, beginning with the most widely used “high production volume” (HPV) chemicals. REACH testing requirements for the roughly 3000 HPV chemicals specify three separate tests for reproductive toxicity: two developmental toxicity studies on different animal species (OECD Test Guideline 414) and a two-generation reproduction toxicity study (OECD TG 416). These studies are highly costly in both economic and animal welfare terms. OECD TG 416 is a fertility study intended to evaluate reproductive performance of animals …

The Fine-Scale Habitat Use Of Risso’S Dolphins Off Bardsey Island, Cardigan Bay (Uk), Marijke N. De Boer, Sonja Eisfeld, Mark P. Simmonds

Mark P. Simmonds, OBE

The main objectives of this study were (1) to estimate the population size of Risso’s dolphins off Bardsey Island using mark-recapture techniques (De Boer et al., 2013); and (2) to study habitat-use in relation to fine-scale oceanographic features. This work provides preliminary information on the habitat-use of Risso’s dolphins and will benefit future studies, along with the development of effective conservation measures for this species throughout the region.

Cetaceans And Marine Debris: The Great Unknown, Mark Peter Simmonds

Mark P. Simmonds, OBE

Plastics and other marine debris have been found in the gastrointestinal tracts of cetaceans, including instances where large quantities of material have been found that are likely to cause impairment to digestive processes and other examples, where other morbidity and even death have resulted. In some instances, debris may have been ingested as a result of the stranding process and, in others, it may have been ingested when feeding. Those species that are suction or “ram” feeders may be most at risk. There is also evidence of entanglement of cetaceans in marine debris. However, it is usually difficult to distinguish …

Interactions Between Short-Beaked Common Dolphin (Delphinus Delphis) And The Winter Pelagic Pair-Trawl Fishery Ff Southwest England (Uk), Marijke N. De Boer, James T. Saulino, Mardik F. Leopold, Peter J.H. Reijnders, Mark P. Simmonds

Mark P. Simmonds, OBE

During offshore and onshore studies (2004 to 2009), the interactions between pair-trawls and short-beaked common dolphins (Delphinus delphis) were studied to better understand the impact of bycatch. A ‘hotspot’ area where pair-trawls overlapped with high dolphin abundance was identified. We made comparisons between boat-based data collected in absence and presence of pair-trawlers. The relative abundance and group-size of dolphins was significantly higher in the presence of pair-trawlers. Dolphins were observed associating with towing and hauling procedures. Significantly, more carcasses occurred in areas with hauling-activity than those without. Body-temperatures obtained from carcasses found near operating pair-trawlers indicated that bycatch mostly occurred …

Health Professionals’ Roles In Animal Agriculture, Climate Change, And Human Health, Aysha Z. Akhtar, Michael Greger, Hope Ferdowsian, Erica Frank

Michael Greger, MD, FACLM

What we eat is rapidly becoming an issue of global concern. With food shortages, the rise in chronic disease, and global warming, the impact of our dietary choices seems more relevant today than ever. Globally, a transition is taking place toward greater consumption of foods of animal origin, in lieu of plantbased diets. With this transition comes intensification of animal agriculture that in turn is associated with the emergence of zoonotic infectious diseases, environmental degradation, and the epidemics of chronic disease and obesity. Health professionals should be aware of these trends and consider them as they promote healthier and more …

Volunteer Studies In Pain Research — Opportunities And Challenges To Replace Animal Experiments: The Report And Recommendations Of A Focus On Alternatives Workshop, C. K. Langley, Q. Aziz, C. Bountra, N. Gordon, P. Hawkins, A. Jones, G. Langley, T. Nurmikko, I. Tracey

Gill Langley, PhD

Despite considerable research, effective and safe treatments for human pain disorders remain elusive. Understanding the biology of different human pain conditions and researching effective treatments continue to be dominated by animal models, some of which are of limited value. British and European legislation demands that non-animal approaches should be considered before embarking on research using experimental animals. Recent scientific and technical developments, particularly in human neuroimaging, offer the potential to replace some animal procedures in the study of human pain. A group of pain research experts from academia and industry met with the aim of exploring creatively the tools, strategies …

The Validity Of Animal Experiments In Medical Research, Gill Langley

Gill Langley, PhD

Other animals, such as mice, rats, rabbits, dogs and monkeys, are widely used as surrogates for humans in fundamental medical research. This involves creating disorders in animals by chemical, surgical or genetic means, with the aim of mimicking selected aspects of human illnesses. It is a truism that any model or surrogate is not identical to the target being modelled. So, in medical research, experiments using animals or cell cultures or even healthy volunteers instead of patients (being the target population with the target illness) will inevitably have limitations, although these will be greater or lesser depending on the model.

Conversion Of Red Fluorescent Protein Into A Bright Blue Probe, Oksana M. Subach, Illia S. Gundorov, Masami Yoshimura, Fedor V. Subach, Jinghang Zhang, David Grunwald, Ekaterina A. Souslova, Dmitriy M. Chudakov, Vladislav V. Verkhusha

David Grünwald

We used a red chromophore formation pathway, in which the anionic red chromophore is formed from the neutral blue intermediate, to suggest a rational design strategy to develop blue fluorescent proteins with a tyrosine-based chromophore. The strategy was applied to red fluorescent proteins of the different genetic backgrounds, such as TagRFP, mCherry, HcRed1, M355NA, and mKeima, which all were converted into blue probes. Further improvement of the blue variant of TagRFP by random mutagenesis resulted in an enhanced monomeric protein, mTagBFP, characterized by the substantially higher brightness, the faster chromophore maturation, and the higher pH stability than blue fluorescent proteins …

Tal Effector-Nucleotide Targeter (Tale-Nt) 2.0: Tools For Tal Effector Design And Target Prediction, Erin L. Doyle, Nicholas J. Booher, Daniel S. Standage, Daniel F. Voytas, Volker P. Brendel, John K. Vandyk, Adam J. Bogdanove

John K. VanDyk

Transcription activator-like (TAL) effectors are repeat-containing proteins used by plant pathogenic bacteria to manipulate host gene expression. Repeats are polymorphic and individually specify single nucleotides in the DNA target, with some degeneracy. A TAL effector-nucleotide binding code that links repeat type to specified nucleotide enables prediction of genomic binding sites for TAL effectors and customization of TAL effectors for use in DNA targeting, in particular as custom transcription factors for engineered gene regulation and as site-specific nucleases for genome editing. We have developed a suite of web-based tools called TAL Effector-Nucleotide Targeter 2.0 (TALE-NT 2.0;https://boglab.plp.iastate.edu/) that enables design of custom …

Sound And The Moving Image:Critical Characteristics For Spectator Response, Kara Rader, Rafeeq I. Roberts, Matt Egizii, Jeffery Allen

Rafeeq I Roberts

The prevailing wisdom in the film and video production industries is that audio information outweighs visual information when it comes to spectator responses, but there are few empirical studies to support this claim. In previous research, four critical characteristics of sound have been identified: (1) Music, (2) visual/sound contradictions (defined as sound that is inconsistent with audience expectations based on visual information), (3) multi-channel sound, and (4) sound quality. Building on our previous research into music and film, we have found that many researchers have looked into the question of how music affects emotions (Eschrich et al., 2008; Have, 2008; …

Effectiveness Of A Novel Spontaneous Carvacrol Nanoemulsion Against Salmonella Enterica Enteritidis And Escherichia Coli O157:H7 On Contaminated Mung Bean And Alfalfa Seeds, Kyle Landry, Yuhua Chang, David Julian Mcclements, Lynne Mclandsborough

Kyle S Landry

Pubertal And Adult Leydig Cell Function In Mullerian Inhibiting Substance-Deficient Mice, Xiufeng Wu, Ramamani Arumugam, Stephen Baker, Mary Lee

Mary M. Lee

Mullerian inhibiting substance (MIS) causes Mullerian duct regression during sexual differentiation and regulates postnatal Leydig cell development. MIS knockout (MIS-KO) mice with targeted deletions of MIS develop Leydig cell hyperplasia, but their circulating androgen concentrations are reportedly unaltered. We compared reproductive hormone profiles, androgen biosynthesis, and the expression of key steroidogenic and metabolic enzymes in MIS-KO and wild-type (WT) mice at puberty (36 d) and sexual maturity (60 d). In pubertal animals, basal testosterone and LH concentrations in plasma were lower in MIS-KO than WT mice, whereas human chorionic gonadotropin-stimulated testosterone concentrations were similar. In adults, basal LH, and both …

The Ups And Downs Of Knowledge Infrastructures In Science: Implications For Data Management, Christine L. Borgman, Peter T. Darch, Ashley E. Sands, Jillian C. Wallis, Sharon Traweek

Christine L. Borgman

The promise of technology-enabled, data-intensive scholarship is predicated upon access to knowledge infrastructures that are not yet in place. Scientific data management requires expertise in the scientific domain and in organizing and retrieving complex research objects. The Knowledge Infrastructures project compares data management activities of four large, distributed, multidisciplinary scientific endeavors as they ramp their activities up or down; two are big science and two are small science. Research questions address digital library solutions, knowledge infrastructure concerns, issues specific to individual domains, and common problems across domains. Findings are based on interviews (n=113 to date), ethnography, and other analyses of …

Heterogeneous Rotational Diffusion Of A Fluorescent Probe In Lipid Monolayers, Christina M. Othon

Christina M Othon

The rotational correlation time of the lipid probe 1-palmitoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine (NBD-PC) is measured using fluorescence anisotropy for two lipid species. We measure the rotational diffusion in a monolayer of 1,2-Didecanoyl-sn-glycero-3-phosphocholine (DPPC) which displays a phase transition at room temperature from the liquid expanded to the liquid-condensed phase. The constant rotational diffusion of the probe throughout the phase transition reflects the measurement of dynamics in only the liquid-expanded phase. We contrast the dynamic changes during this phase coexistence to the continuous density increase observed in 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at room temperature. We observe a non-exponential decay of the probe diffusion consistent with heterogeneity …

Evidence Of Enso Mega-Drought Triggered Collapse Of Prehistory Aboriginal Society In Northwest Australia, Hamish Mcgowan, Samuel K. Marx, P Moss, Andrew Hammond

Samuel K Marx

The Kimberley region of northwest Australia contains one of the World's largest collections of rock art characterised by two distinct art forms; the fine featured anthropomorphic figures of the Gwion Gwion or Bradshaw paintings, and broad stroke Wandjina figures. Luminescence dating of mud wasp nests overlying Gwion Gwion paintings has confirmed an age of at least 17,000 yrs B.P. with the most recent dates for these paintings from around the mid-Holocene (5000 to 7000 yrs B.P.). Radiocarbon dating indicates that the Wandjina rock art then emerged around 3800 to 4000 yrs B.P. following a hiatus of at least 1200 yrs. …

Dust Transport And Deposition In A Superhumid Environment, Samuel K. Marx, Hamish A. Mcgowan

Samuel K Marx

Contemporary rates of dust deposition monitored along a 300-km section of the superhumid West Coast of New Zealand's South Island are presented. In this setting, dust is entrained primarily from the dry channels of braided glaciofluvial rivers that drain the western slopes of the Southern Alps. Measured dust deposition ranged between 0.21 and 118.9 kg-1 ha -1 month-1, which is similar to dust deposition rates monitored in arid and semiarid environments. However, these are not considered to be sufficient for present day loess genesis. Dust deposition was highest in summer because of the greater frequency of favourable dust transporting winds, …

Evidence Of Solar And Tropical-Ocean Forcing Of Hydroclimate Cycles In Southeastern Australia For The Past 6500 Years, Hamish A. Mcgowan, Samuel K. Marx, Joshua Soderholm, John Denholm

Samuel K Marx

Evidence of solar and tropical‐ocean forcing of climate cycles has been found in numerous palaeoclimate records. Numerical modelling studies show physical mechanisms by which direct and indirect solar forcing may affect climate, while there is mounting evidence of solar forcing of tropical ocean‐atmosphere teleconnections. This study has developed a 6500 year record of dust deposition, a proxy for regional hydroclimate variability for the Snowy Mountains region of Australia. Spectral analysis of the record provides evidence of statistically significant cycles in dust deposition of 35–43 years, 62–73 years, 161 years and 2200 years. These correlate with variability in solar irradiance and …

Characterization Of A Recently Purified Thermophilic Dnase From A Novel Thermophilic Fungus, Kyle Landry, Robert Levin

Kyle S Landry

Taphonomy Of The Joanna Track Site, Cretaceous Glen Rose Formation: Is The Shrimp Mightier Than The Dinosaur?, Michael Blair, Benjamin Dattilo, Lydia Mark, James Farlow, Jerry Jacene, Shelley Mcfadin

Benjamin F. Dattilo

Dinosaur trackways are common in the Cretaceous Glen Rose Formation of central Texas. While the trackways in Dinosaur Valley State Park are well known, many other sites can be found in this region. Recently, a new track site was discovered during construction in Glen Rose, Texas. The Joanna Track Site features tridactyl tracks which have been partly obliterated by invertebrates burrowing through the thick mud which buried them.

We measured and described the interval from 0.3 m below the track layer through 2.7 m above it in a vertical outcrop directly adjacent to the track site. Samples were collected in …

Microstratigraphic Analysis Of Burrow-Reworked Dinosaur Track Bed At Joanna's Track Site, Cretaceous Glen Rose Formation, Glen Rose, Texas, Michael Blair, Benjamin Dattilo, Anthony Martin, James Farlow

Benjamin F. Dattilo

Although dinosaur trackways are common in the Cretaceous Glen Rose Formation of Texas, the recently discovered Joanna track site illustrates a unique ichnological relationship where dinosaur tracks were disrupted by invertebrate burrows made long after burial. In an effort to document the precise sequence of events, we described the interval from 0.3 m below the track layer through 2.7 m above it in a vertical outcrop adjacent to the track site, focusing on the 70-cm of strata immediately above the track horizon. An 8-meter N-S cross-section of this 70-cm interval was power-washed, examined for trace fossils, body fossils, and lithology …

Push Me – Pull You: Experimental Biomechanics Of Immobile Suspension Feeders On Soft Substrates, Roy Plotnick, Benjamin Dattilo, Joshua Corrie, Daniel Piquard, Jennifer Bauer

Benjamin F. Dattilo

Immobile suspension feeders on soft substrates (ISOSS; Thayer 1979) although rare in modern marine habitats, were relatively common in the Paleozoic. Numerous Paleozoic taxa have been interpreted as dwelling on soft unconsolidated sediments and possessing morphologic features that either anchor them to the sea floor (e.g., crinoid holdfasts) or prevent them from sinking in (strophomenid brachiopods). Thayer (1975) reviewed the morphologic adaptations for forms living on soft-muddy bottoms and provided a quantitative expression of the static stresses involved. The same quantitative expression can also be used to describe the forces involved in anchoring. With the exception of Leighton and Savarese …

An Unusual Association Of Pseudolingula And Rafinesquina From The Upper Ordovician Of Ohio, Benjamin Dattilo, Rebecca Freeman, Bryan Utesch, Steve Felton, John Pojeta

Benjamin F. Dattilo

Late Ordovician members of Order Lingulida, for the most part, resemble modern lingulids in their infaunal habits and marginal habitats. Pseudolingula, a common Cincinnatian form, is often found preserved in burrows in life position, and as such, it could probably escape moderate sediment accumulations. An unusual association of thousands of specimens of the lingulate Pseudolingula and hundreds of the strophomenid Rafinesquina in the Upper Ordovician of the Cincinnati, Ohio region presents an interesting case. This association occurs on 4-square-meter exposure of a 10 cm shell bed in the Fairview Formation at Harsha Lake, Ohio. The bed is covered with Rafinesquina …

Dinosaur Tracksites Of The Paluxy River Valley (Glen Rose Formation, Lower Cretaceous), Dinosaur Valley State Park, Somervell County, Texas., James O. Farlow, Mike O'Brien, Glenn J. Kuban, Benjamin F. Dattilo, K. T. Bates, Peter L. Falkingham, L. Pinuela, Amanda Rose, A. Freels, C. Kumagai, Courtney Libben, Justin Smith, J. Whitcraft

Benjamin F. Dattilo

In 1940 R.T. Bird of the American Museum of Natural History collected segments of a sauropod and a theropod trackway from a site in the bed (Glen Rose Formation; Lower Cretaceous) of the Paluxy River, in what is now Dinosaur Valley State Park (Glen Rose, Texas, USA). However, Bird left undocumented thousands of other dinosaur footprints from this and other Paluxy tracksites. In 2008 and 2009 our international team carried out fieldwork to create detailed photomosaics of extant Paluxy tracksites, using GIS technology to combine these with historic maps and photographs. We also made photographs, tracings, LiDAR images, and measurements …

What Can We Learn From Confusing Olivella Columellaris And O. Semistriata (Olivellidae, Gastropoda), Two Key Species In Panamic Sandy Beach Ecosystems?, Allison I. Troost, Samantha D. Rupert, Ariel Z. Cyrus, Frank V. Paladino, Benjamin F. Dattilo, Winfried S. Peters

Benjamin F. Dattilo

Olivella columellaris (Sowerby 1825) and O. semistriata (Gray 1839) are suspension-feeding, swash-surfing snails on tropical sandy beaches of the east Pacific. While they often are the numerically dominant macrofaunal element in their habitats, their biology is poorly understood; the two species actually have been confused in all of the few publications that address their ecology. Frequent misidentifications in publications and collections contributed also to an overestimation of the geographic overlap of the two species. To provide a sound taxonomic basis for further functional, ecological, and evolutionary investigations, we evaluated the validity of diagnostic traits in wild populations and museum collections, …

The Curse Of Rafinesquina: Negative Taphonomic Feedback Exerted By Strophomenid Shells On Storm-Buried Lingulids In The Cincinnatian (Katian, Ordovician) Series Of Ohio, Rebecca Freeman, Benjamin Dattilo, Aaron Morse, Michael Blair, Steve Felton, John Pojeta

Benjamin F. Dattilo

Thousands of lingulid brachiopods were found clustered beneath hundreds of individual valves of the strophomenid brachiopod Rafinesquina in the Upper Ordovician of Ohio. This association suggested a relationship between the two brachiopods, but the nature of this relationship was unclear. We utilized serial thin sectioning to examine these brachiopods and to determine the origin of the bed in which they were found. Sedimentary structures, mixed taphonomies, and stratigraphic and paleogeographic setting suggest that the lingulids occupied a hiatal concentration that had previously been reworked, but not significantly transported, by tropical storms. The final burial event was a storm that exhumed …

The “Passive Implanter” Strategy Of The Adult Ordovician Brachiopod, Platystrophia Ponderosa., Sadye Howald, Benjamin Dattilo

Benjamin F. Dattilo

Platystrophia ponderosa is found throughout the Maysvillian Strata of the Cincinnati Ordovician. This species thrived in a high energy environment with only muddy shell gravels, and no solid substrates for pedicle attachment. Our growth-series studies show juveniles of this species had large pedicle openings, thin shell, small size, nearly flat shape, and shallow sinus/sulcus. In contrast, the adults had relatively small pedicle openings obstructed by a large beak, secondary thickening of the pedicle valve making it considerably thicker than the brachial valve, large size (up to 4cm in diameter), spherical shape, and deep sinus/sulcus. The morphological characteristics of the adult …

How Many Track Horizons Are Exposed At Dinosaur Valley State Park? Stratigraphy Of The Cretaceous Glen Rose Formation Track Sites Of The Paluxy River, Glen Rose, Texas, Benjamin Dattilo, Sadye Howald, James Farlow, Anthony Martin

Benjamin F. Dattilo

The dinosaur tracks of the Glen Rose Formation in the Paluxy River at Dinosaur Valley State Park are among the best preserved and most abundant in the world. While many track sites are easily correlated to the Main Tracksite, others, especially those at the extreme ends of the park, are differently preserved and not obviously correlated. To count track horizons, several stratigraphic sections were measured along the river from upstream at the McFall Ledge Site to 7.6 km downstream at the County Road 1001 crossing (3.1 km linear distance). These reveal 6 meters of strata separating two track-bearing horizons exposed …