Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Discipline
- Keyword
- File Type
Articles 1 - 5 of 5
Full-Text Articles in Physical Sciences and Mathematics
Concentration Of Atmospheric Ammonia From Kenyon College Monitoring Station, Vivian Ezeh, David Heithaus, Simon Garcia, Matthew F. Rouhier
Concentration Of Atmospheric Ammonia From Kenyon College Monitoring Station, Vivian Ezeh, David Heithaus, Simon Garcia, Matthew F. Rouhier
Matthew F Rouhier
Kenyon College is hosting an ammonia (NH3) monitoring station as part of the National Atmospheric Deposition Program (NADP). The Kenyon College (OH32) site is one of six locations around the state of Ohio.
Pharmacological Validation Of An Inward-Rectifier Potassium (Kir) Channel As An Insecticide Target In The Yellow Fever Mosquito Aedes Aegypti, Matthew F. Rouhier, Rene Raphemot, Jerod S. Denton, Peter M. Piermarini
Pharmacological Validation Of An Inward-Rectifier Potassium (Kir) Channel As An Insecticide Target In The Yellow Fever Mosquito Aedes Aegypti, Matthew F. Rouhier, Rene Raphemot, Jerod S. Denton, Peter M. Piermarini
Matthew F Rouhier
Mosquitoes are important disease vectors that transmit a wide variety of pathogens to humans, including those that cause malaria and dengue fever. Insecticides have traditionally been deployed to control populations of disease-causing mosquitoes, but the emergence of insecticide resistance has severely limited the number of active compounds that are used against mosquitoes. Thus, to improve the control of resistant mosquitoes there is a need to identify new insecticide targets and active compounds for insecticide development. Recently we demonstrated that inward rectifier potassium (Kir) channels and small molecule inhibitors of Kir channels offer promising new molecular targets and active compounds, respectively, …
Discovery And Characterization Of A Potent And Selective Inhibitory Of Aedes Aegypti Inward Rectifier Potassium Channels, Matthew F. Rouhier, Rene Raphemot, Daniel R. Swale, Emily Days, C. David Weaver, Kimberly M. Lovell, Leah C. Konkel, Darren W. Engers, Sean F. Bollinger, Corey Hopkins, Peter M. Piermarini, Jerod S. Denton
Discovery And Characterization Of A Potent And Selective Inhibitory Of Aedes Aegypti Inward Rectifier Potassium Channels, Matthew F. Rouhier, Rene Raphemot, Daniel R. Swale, Emily Days, C. David Weaver, Kimberly M. Lovell, Leah C. Konkel, Darren W. Engers, Sean F. Bollinger, Corey Hopkins, Peter M. Piermarini, Jerod S. Denton
Matthew F Rouhier
Vector-borne diseases such as dengue fever and malaria, which are transmitted by infected female mosquitoes, affect nearly half of the world's population. The emergence of insecticide-resistant mosquito populations is reducing the effectiveness of conventional insecticides and threatening current vector control strategies, which has created an urgent need to identify new molecular targets against which novel classes of insecticides can be developed. We previously demonstrated that small molecule inhibitors of mammalian Kir channels represent promising chemicals for new mosquitocide development. In this study, high-throughput screening of approximately 30,000 chemically diverse small-molecules was employed to discover potent and selective inhibitors of Aedes …
Eliciting Renal Failure In Mosquitoes With A Small-Molecule Inhibitor Of Inward-Rectifying Potassium Channels, Matthew F. Rouhier, Rene Raphemot, Corey R. Hopkins, Rocco D. Gogliotti, Kimberly M. Lovel, Rebecca M. Hine, Dhairyasheel Ghosalkar, Anthony Longo, Klaus W. Beyenbach, Jerod S. Denton, Peter M. Piermarini
Eliciting Renal Failure In Mosquitoes With A Small-Molecule Inhibitor Of Inward-Rectifying Potassium Channels, Matthew F. Rouhier, Rene Raphemot, Corey R. Hopkins, Rocco D. Gogliotti, Kimberly M. Lovel, Rebecca M. Hine, Dhairyasheel Ghosalkar, Anthony Longo, Klaus W. Beyenbach, Jerod S. Denton, Peter M. Piermarini
Matthew F Rouhier
Mosquito-borne diseases such as malaria and dengue fever take a large toll on global health. The primary chemical agents used for controlling mosquitoes are insecticides that target the nervous system. However, the emergence of resistance in mosquito populations is reducing the efficacy of available insecticides. The development of new insecticides is therefore urgent. Here we show that VU573, a small-molecule inhibitor of mammalian inward-rectifying potassium (Kir) channels, inhibits a Kir channel cloned from the renal (Malpighian) tubules of Aedes aegypti (AeKir1). Injection of VU573 into the hemolymph of adult female mosquitoes (Ae. aegypti) disrupts the production and excretion of urine …
Converting Glx2-1 Into An Active Glyoxalase Ii, Pattraranee Limphong, Nicole Adams, Matthew Rouhier, Ross Mckinney, Melissa Naylor, Brian Bennett, Christopher Makaroff, Michael Crowder
Converting Glx2-1 Into An Active Glyoxalase Ii, Pattraranee Limphong, Nicole Adams, Matthew Rouhier, Ross Mckinney, Melissa Naylor, Brian Bennett, Christopher Makaroff, Michael Crowder
Matthew F Rouhier
Arabidopsis thaliana glyoxalase 2-1 (GLX2-1) exhibits extensive sequence similarity with GLX2 enzymes but is catalytically inactive with SLG, the GLX2 substrate. In an effort to identify residues essential for GLX2 activity, amino acid residues were altered at positions 219, 246, 248, 325, and 328 in GLX2-1 to be the same as those in catalytically active human GLX2. The resulting enzymes were overexpressed, purified, and characterized using metal analyses, fluorescence spectroscopy, and steady-state kinetics to evaluate how these residues affect metal binding, structure, and catalysis. The R246H/N248Y double mutant exhibited low level S-lactoylglutathione hydrolase activity, while the R246H/N248Y/Q325R/R328K mutant exhibited a …