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Full-Text Articles in Physical Sciences and Mathematics
Comparing Authentic And Cryptic 5’ Splice Sites Using Hidden Markov Models And Decision Trees, Pratikshya Mishra
Comparing Authentic And Cryptic 5’ Splice Sites Using Hidden Markov Models And Decision Trees, Pratikshya Mishra
Master's Projects
Splicing is the editing of the precursor mRNA produced during transcription. The mRNA contains a large number of nucleotides in the introns and exons which are spliced to remove the introns and bind the exons to produce the mature mRNA which is translated to generate proteins. Hence accurate splicing at 5’ and 3’ splice sites (authentic splice sites (AuthSS)) is of foremost importance. The 5’ and 3’ splice sites are characterized by consensus sequences. Eukaryotic genome also contains splice sites known as Cryptic Splice Sites (CSS) that match the consensus. But the CSS are activated only when there is a …
Computational Analysis Of Cryptic Splice Sites, Remya Mohanan
Computational Analysis Of Cryptic Splice Sites, Remya Mohanan
Master's Projects
DNA in the nucleus of all eukaryotes is transcribed into mRNA where it is then translated into proteins. The DNA which is transcribed into mRNA is composed of coding and non-coding regions called exons and introns, respectively. It undergoes a post-trancriptional process called splicing where the introns or the non-coding regions are removed from the pre-mRNA to give the mature mRNA. Splicing of pre-mRNAs at 5 ́ and 3ˊ ends is a crucial step in the gene expression pathway. The mis-splicing by the spliceosome at different sites known as cryptic splice sites is caused by mutations which will affect the …