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Full-Text Articles in Physical Sciences and Mathematics
Protein Lc-Ms Using Slip Flow Chromatography, Ximo Zhang
Protein Lc-Ms Using Slip Flow Chromatography, Ximo Zhang
Open Access Dissertations
Histones are essential chromosomal proteins with large numbers of variants and post-translational modifications (PTMs). PTM levels of histones are known to correlate with different stages of cancer. Due to the lack of resolution on intact histone separations, conventional methods for histone analysis require time-consuming digestion, which often leads to the loss of PTM information. Slip flow chromatography with orderly packed nonporous silica particles has been shown to greatly increase the efficiency of protein separation by reducing eddy diffusion and resistance to mass transfer. In this work, higher resolution for intact histones separation was achieved by using a 5 cm capillary …
Enhancing Silymarin Fractionation Via Molecular Modeling Using The Conductor-Like Screening Model For Real Solvents, Emma C. Brace
Enhancing Silymarin Fractionation Via Molecular Modeling Using The Conductor-Like Screening Model For Real Solvents, Emma C. Brace
Open Access Theses
The market for bio-based products from plant sources is on the rise. There is a global challenge to implement environmentally clean practices for the production of fuels and pharmaceuticals from sustainable resources. A significant hurdle for discovery of comparable plant-derived products is the extensive volume of trial-and-error experimentation required. To alleviate the experimental burden, a quantum mechanics based molecular modeling approach known as the COnductor-like Screening Model for Real Solvents (COSMO-RS) was used to predict the best biphasic solvent system to purify silymarins from an aqueous mixture. Silymarins are a class of flavonolignans present in milk thistle ( Silybum marianum …