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Full-Text Articles in Physical Sciences and Mathematics
Isolation Of Diverse Members Of The Aquificales From Geothermal Springs In Tengchong, China, Brian P. Hedlund, Anna-Louise Reysenbach, Liuquin Huang, John C. Ong, Zizhang Liu, Jeremy A. Dodsworth, Reham Ahmed, Amanda J. Williams, Brandon R. Briggs, Yitai Liu, Weiguo Hou, Hailiang Dong
Isolation Of Diverse Members Of The Aquificales From Geothermal Springs In Tengchong, China, Brian P. Hedlund, Anna-Louise Reysenbach, Liuquin Huang, John C. Ong, Zizhang Liu, Jeremy A. Dodsworth, Reham Ahmed, Amanda J. Williams, Brandon R. Briggs, Yitai Liu, Weiguo Hou, Hailiang Dong
Biology Faculty Publications and Presentations
The order Aquificales (phylum Aquificae) consists of thermophilic and hyperthermophilic bacteria that are prominent in many geothermal systems, including those in Tengchong, Yunnan Province, China. However, Aquificales have not previously been isolated from Tengchong. We isolated five strains of Aquificales from diverse springs (temperature 45.2–83.3°C and pH 2.6–9.1) in the Rehai Geothermal Field from sites in which Aquificales were abundant. Phylogenetic analysis showed that four of the strains belong to the genera Hydrogenobacter, Hydrogenobaculum, andSulfurihydrogenibium, including strains distant enough to likely justify new species ofHydrogenobacter and Hydrogenobaculum. The additional strain may represent a …
Microbial Biogeochemistry Of Boiling Springs Lake: A Physically Dynamic, Oligotrophic, Low-P H Geothermal Ecosystem, Patricia Lynne Siering, Gordon V. Wolfe, Mark S. Wilson, A. N. Yip, Cynthia M. Carey, Colin D. Wardman, Russell Scott Shapiro, Kenneth M. Stedman, Jennifer E. Kyle, Tong Yuan, Joy D. Van Nostrand, Zhili He, Jizhong Zhou
Microbial Biogeochemistry Of Boiling Springs Lake: A Physically Dynamic, Oligotrophic, Low-P H Geothermal Ecosystem, Patricia Lynne Siering, Gordon V. Wolfe, Mark S. Wilson, A. N. Yip, Cynthia M. Carey, Colin D. Wardman, Russell Scott Shapiro, Kenneth M. Stedman, Jennifer E. Kyle, Tong Yuan, Joy D. Van Nostrand, Zhili He, Jizhong Zhou
Biology Faculty Publications and Presentations
Boiling Springs Lake (BSL) in Lassen Volcanic National Park, California, is North America's largest hot spring, but little is known about the physical, chemical, and biological features of the system. Using a remotely operated vessel, we characterized the bathymetry and near-surface temperatures at sub-meter resolution. The majority of the 1.2 ha, pH 2.2 lake is 10 m deep and 50-52 [degrees]C, but temperatures reach 93 [degrees]C locally. We extracted DNA from water and sediments collected from warm (52 [degrees]C) and hot (73-83 [degrees]C) sites separated by 180 m. Gene clone libraries and functional gene microarray (GeoChip 3.0) were used to …
Virus Silicification Under Simulated Hot Spring Conditions, James R. Laidler, Kenneth M. Stedman
Virus Silicification Under Simulated Hot Spring Conditions, James R. Laidler, Kenneth M. Stedman
Biology Faculty Publications and Presentations
Silicification of organisms in silica-depositing environments can impact both their ecology and their presence in the fossil record. Although microbes have been silicified under laboratory and environmental conditions, viruses have not. Bacteriophage T4 was successfully silicified under laboratory conditions that closely simulated those found in silica-depositing hot springs. Virus morphology was maintained, and a clear elemental signature of phosphorus was detected by energy-dispersive X-ray spectrophotometry (EDS).