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Full-Text Articles in Physical Sciences and Mathematics

Benthic Community Dynamics And Stony Coral Demographics In Florida In Relation To Acute Disturbances And Chronic Pressures, Nicholas P. Jones Aug 2022

Benthic Community Dynamics And Stony Coral Demographics In Florida In Relation To Acute Disturbances And Chronic Pressures, Nicholas P. Jones

All HCAS Student Capstones, Theses, and Dissertations

The persistence of coral reef communities is threatened by a suite of pressures operating at varying spatial and temporal scales. In general, acute disturbances (short term stochastic events such as marine heatwaves or hurricanes) and chronic pressures, such as ocean warming, have caused the most significant changes to stony coral assemblages (order Scleractinia) and continue to impair recovery potential. Additionally, many coral reefs are subject to local chronic anthropogenic pressures resulting in poor water quality or sedimentation, which further impact stony corals and shape benthic community structure, particularly near urbanized coastlines. For the viability of communities on coral reefs, a …


Next Generation Sequencing Methods For Coastal Zone Water Quality Monitoring, Catherine Iskrenko Aug 2018

Next Generation Sequencing Methods For Coastal Zone Water Quality Monitoring, Catherine Iskrenko

HCNSO Student Capstones

When analyzing the water quality of the coastal zone, culture-based techniques have been utilized most often to identify Fecal Indicator Bacteria in samples. Since the advent of the Sanger Method for DNA sequencing, other techniques have arisen that provide significantly more information on the microorganisms in sample, but they are still not the mainstream for water quality analysis. This capstone reviews and compares culture-based techniques, DNA sequencing, RNA sequencing, qPCR for biomarker, and 16S rDNA sequencing to highlight their merits and shortcomings for analyzing environmental water samples. The technique presented that provides the broadest range of information (including the identification …


Bacterial Indicators Of Fecal Pollution: Exploring Relationships Between Fecal Coliform And Enterococcus Groups In Central And South Florida Surface Waters, Shelby G. Craig Mar 2016

Bacterial Indicators Of Fecal Pollution: Exploring Relationships Between Fecal Coliform And Enterococcus Groups In Central And South Florida Surface Waters, Shelby G. Craig

HCNSO Student Theses and Dissertations

Ambient and recreational surface waters worldwide experience fecal pollution due to a variety of anthropogenic sources. Fecal waste has been proven, for over a century, to harbor pathogenic microorganisms which subsequently cause a variety of disease and illness in human hosts. The benefits of utilizing fecal indicator bacteria (FIB) as a simple, inexpensive means to detect fitful human pathogens within a variety of water matrices are vast. However, no universal agreement exists in regard to which indicator is best suited for detection of fecal contamination and pathogens in environmental waters, and no single standard for bacterial indicators has been federally …