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Physical Sciences and Mathematics Commons

Open Access. Powered by Scholars. Published by Universities.®

Missouri University of Science and Technology

2011

Life Sciences

Denaturing gradient gel electrophoresis (DDGE)

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Isolation And Characterization Of Bacterial Symbionts From Crotalaria Spectabilis Grown On Trichloroethene Contaminated Soil, April Lyn Rocha Jan 2011

Isolation And Characterization Of Bacterial Symbionts From Crotalaria Spectabilis Grown On Trichloroethene Contaminated Soil, April Lyn Rocha

Masters Theses

"The discovery of several members of the Leguminosae family growing on a TCE contaminated site demonstrated that they have the capabilities to tolerate the TCE contamination. This led to an investigation of the plants' ability to metabolize TCE. Research by Dr. Lee Newman showed that nodules from Crotalaria spectabilis grown on the site had TCE metabolites present while nodules from other legumes at the site did not. The presence of TCE metabolites within the C. spectabilis nodules suggests that the bacterial symbiont may be responsible for TCE metabolism. This led us to investigate the symbiont of C. spectabilis and its …