Physical Sciences and Mathematics Commons^™

A Critical Inter-Subunit Interaction For The Transmission Of The Allosteric Signal In The Agrobacterium Tumefaciens Adp-Glucose Pyrophosphorylase, Hiral Patel, Gabriela Martinez-Ramirez Ms., Emily Dobrzynski, Alberto A. Iglesias, Dali Liu, Miguel Ballicora

Chemistry: Faculty Publications and Other Works

ADP-glucose pyrophosphorylase is a key regulatory enzyme involved in starch and glycogen synthesis in plants and bacteria, respectively. It has been hypothesized that inter-subunit communications are important for the allosteric effect in this enzyme. However, no specific interactions have been identified as part of the regulatory signal. The enzyme from Agrobacterium tumefaciens is a homotetramer allosterically regulated by fructose 6-phosphate and pyruvate. Three pairs of distinct subunit-subunit interfaces are present. Here we focus on an interface that features two symmetrical interactions between Arg11 and Asp141 from one subunit with residues Asp141 and Arg11 of the neighbor subunit, respectively. Previously, scanning …