Physical Sciences and Mathematics Commons^™

Non-Target Analysis Using High-Resolution Mass Spectrometry To Characterize And Remediate Urban Waters, Brian Ng

FIU Electronic Theses and Dissertations

The first part of this dissertation will focus on the development of a simple, robust online solid phase extraction liquid chromatography high resolution mass spectrometry (SPE LC-HRMS) method followed by the use of computational software workflows for non-target analysis (NTA) of environmental samples (development). The benchmarks to assess reproducibility are not well defined for non-target analysis. Parameters to evaluate analytical performance, such as accuracy, precision and selectivity, are well defined for target analysis, but remain elusive for non-target screening analysis. In this study, quality control (QC) guidelines are proposed to assure reliable data in NTA methodologies using a simple …

Utilizing Headspace Solid-Phase Microextraction For The Characterization Of Volatile Organic Compounds Released From Contraband And Its Implications For Detector Dog Training, Kelvin Joseph Frank Jr

FIU Electronic Theses and Dissertations

Improving the accuracy and reliability of odor detection dogs is of utmost importance particularly for legal reasons. Field testing in conjunction with headspace analysis of volatile organic compounds (VOCs) has in recent times allowed for these improvements, by providing scientifically based recommendations for optimum training protocols. The current project leveraged on these established capabilities to enhance three areas of odor detection: illicit drugs, explosives and mass storage devices.

With hemp being legalized under the 2018 Farm Bill, legal questions have been raised regarding a dog’s ability to ignore hemp if trained to detect marijuana, as both are types of Cannabis …

Structural Characterization Of Biomolecules Using Tandem Non- Linear And Linear Ion Mobility, Action Spectroscopy, Mass Spectrometry And Molecular Dynamics, Jacob Edward Porter

FIU Electronic Theses and Dissertations

The following thesis comprises the use of novel gas-phase spectroscopic and spectrometric techniques complemented with molecular dynamics for the structural characterization of biomolecules: from small model systems to large proteins. In particular, complementary linear and non-linear ion mobility, mass spectrometry and action spectroscopy has been applied to the separation and characterization of native and kinetic intermediate states of biomolecular ions. This suite of tools, comprising ion mobility in tandem with non-ergodic fragmentation and action spectroscopy, provided unique information on the conformational space, folding and binding dynamics of single stranded DNA, peptides, peptide-cofactor and protein systems. Theoretical modeling provided candidate structures …

Simplifying Sample Preparation Using Fabric Phase Sorptive Extraction: Analysis Of Trace Targeted Pollutant Residues In Environmental, Biological And Food Samples, Rodolfo Mesa

FIU Electronic Theses and Dissertations

Sample preparation is an essential component of analytical methods in chemistry. It is not only necessary but also presents an opportunity to increase the effectiveness of the method significantly. There are various commercially available technologies for sample preparation, including numerous variations of LLE, SPE, and SPME. However, these technologies all present significant deficiencies, including the inability to extract directly from complex samples such as whole milk. Instrumental analysis has been improved greatly in the last two decades but still is not applicable to complex samples without sample preparation.

This work presents the theory of FPSE, including the synthesis of sol-gel …

The Creation And Evaluation Of Novel Canine Training Aids For Cocaine Using Molecularly Encapsulated Sol-Gel Polymers And An Investigation Of Canine Field Accuracy, Michelle Cerreta

FIU Electronic Theses and Dissertations

Biological detectors, such as canines, are valuable tools used for the rapid identification of illicit materials. However, recent increased scrutiny over the reliability, field accuracy, and the capabilities of each detection canine is currently being evaluated in the legal system. For example, the Supreme Court case, State of Florida v. Harris, discussed the need for continuous monitoring of canine abilities, thresholds, and search capabilities. As a result, the fallibility of canines for detection was brought to light, as well as a need for further research and understanding of canine detection. This study is two-fold, as it looks to not only …