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Full-Text Articles in Physical Sciences and Mathematics
Determining Atg11'S Binding Interactions Using A Yeast Two Hybrid System And Directed Mutagenesis, Jesse Smith
Determining Atg11'S Binding Interactions Using A Yeast Two Hybrid System And Directed Mutagenesis, Jesse Smith
Senior Honors Theses and Projects
Autophagy is a mechanism of intracellular degradation within eukaryotes. Because of its aid to a cells longevity, autophagy is believed to be important for the prevention of neurodegenerative diseases. Autophagy related protein 11 (Atg11) is a coiled-coil scaffolding protein required for selective autophagy in yeast. Atg11 is known to interact with Atg1, Atg20, and Atg29, in addition to connecting cargo molecules prApel -Atg19 to the phagophore assembly site. In addition to its many functions within yeast autophagy, Atg11 has two human homologs: Huntingtin protein and FIP200. This homology encourages us to gain a further understanding of Atg11's binding sites. In …
Expression And Purification Of Atg11: A Protein Involved In The Organization Of Autophagy, Carson Zois
Expression And Purification Of Atg11: A Protein Involved In The Organization Of Autophagy, Carson Zois
Senior Honors Theses and Projects
Autophagy is a physiological process for the degradation of internal cell components to promote cell survival. This process allows the cell to maintain homeostasis, recycle damaged cellular components and helps protect against disease. Autophagy can be selective or non-selective. Non-selective, or bulk, autophagy occurs during times of cellular starvation and results in non-specific degradation of cytoplasmic materials. Selective autophagy targets specific components within the cytoplasm for degradation, and is always active. In both forms of autophagy, components are degraded when a double-membraned structure, called the autophagosome, encapsulates the target cargo and is then recruited to the lysosome in mammalian cells, …