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Full-Text Articles in Physical Sciences and Mathematics
Uv Irradiation On Bacteriophage Survival, Sherri Tomlinson
Uv Irradiation On Bacteriophage Survival, Sherri Tomlinson
Honors Theses
Bacteriophage is of great interest because of its potential role in controlling bacterial populations in our environment. UV exposure has a damaging effect on the virus decreasing lytic ability. This study set out to test the effects of UV radiation, in amounts comparable to local environmental conditions, on bacteriophage T2. The virus was placed in a Petri dish in a PBS medium and exposed to UV radiation at 365nm. The irradiated virus was allowed to infect E. coli and plated. The plaques formed were counted to determine lytic activity of the virus with respect to UV irradiation. The results showed …
Rhizocephalan Parasites Of Mud Crabs In South Carolina Estuaries, Erin Burnette
Rhizocephalan Parasites Of Mud Crabs In South Carolina Estuaries, Erin Burnette
Honors Theses
Rhizocephalan parasites often infect commercially important crustacean species such as the blue crab (Callinectes sapidus). In this experiment, the prevalence of rhizocephalan parasites was determined by sampling mud crabs (Panopeus herbstii) in three different locations; Huntington Beach State Park, Waites Island, and Murrells Inlet. Crabs were determined to be parasitized by the presence of an externae extruding from their apron. Unparasitized crabs were also collected to serve as a control group. The externa were removed and DNA extractions were performed. Polymerase chain reactions (PCR) were done to prove whether the crabs were parasitized by Loxothylacus texanus or Loxothylacus panopaei. Results …
Preferential Fish Consumption Based On Omega-3 Fatty Acids And Mercury Concentrations For Maximum Health Benefits, Katrina Smith
Preferential Fish Consumption Based On Omega-3 Fatty Acids And Mercury Concentrations For Maximum Health Benefits, Katrina Smith
Honors Theses
The regular consumption of seafood offers a variety of protective effects, including the reduction of the risk of cardiovascular disease and stroke, due to the presence of omega-3 fatty acids in fish. These protective effects may be diminished by the contamination of seafood by mercury. Mercury increases the risk of cardiovascular problems and impedes neurological development. The objective of this project was to determine the fish species that are appropriate for consumption based on low levels of mercury and recommended intake levels of omega-3 fatty acids. Species that are high in omega-3s and low in mercury include salmon, trout and …
Catalytic Decomposition Of Peroxynitrite And Superoxide By Nafion Films Modified With Iron And Manganese Porphyrin, Amanda Stull, Victor Sarpong
Catalytic Decomposition Of Peroxynitrite And Superoxide By Nafion Films Modified With Iron And Manganese Porphyrin, Amanda Stull, Victor Sarpong
Honors Theses
Nafion films alone and modified with inert electrolytes and metalloporphyrins were tested in their effectiveness of decomposition of aqueous peroxynitrite (PN) and superoxide. Films that were modified with a cationic manganese porphyrin, which has been shown to be responsible for catalytic decomposition of superoxide ion, were found to be most effective. Peroxynitrite was generated in solution by the decomposition of 3-morpholinosydnonimime (SIN-1) that generates nitric oxide and superoxide ion, which rapidly combine to form peroxynitrite. The tyrosine analogue 4-hydroxyphenylacetic (4-HPA) was used to trap the PN in pH 7.00 PBS buffer and was its nitrated product was detected by observing …