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Full-Text Articles in Physical Sciences and Mathematics
Development Of Monolithic Stationary Phases For Cation-Exchange Capillary Liquid Chromatography Of Peptides And Proteins, Xin Chen
Theses and Dissertations
This dissertation focuses on the preparation of polymeric monolithic capillaries for ion exchange chromatography of peptides and proteins, since polymeric monoliths have shown promise for providing improved protein separations. Characteristics of monolithic columns include low back pressure, simplicity of fabrication and biocompatibility. Preparation of strong and weak cation-exchange monolithic stationary phases in 75 μm I.D. capillaries by direct in situ copolymerization was achieved using various functional monomers including sulfopropyl methacrylate, phosphoric acid 2-hydroxyethyl methacrylate, bis[2-(methacryloyloxy)ethyl] phosphate and 2-carboxyethyl acrylate with polyethylene glycol diacrylate and other PEG materials. The resulting monoliths provided excellent ion exchange capillary LC of peptides and proteins …
The Dependence Of The Apparent Pk Of Ion Exchange Celluloses Of Ionic Strength, Paul Cyril Gillespie
The Dependence Of The Apparent Pk Of Ion Exchange Celluloses Of Ionic Strength, Paul Cyril Gillespie
Theses and Dissertations
Diethylaminoethyl cellulose was titrated with acid and base in various concentrations of NaCl. The pK values were determined from titration curves using a graphical technique. Two titratable groups were observed from the acid titration curve, a major and a minor group. The minor group, which represented 20% of the acid titration, was barely detectable in the base titration curve and had a salt-independent pK of 6.0. The pK of the major group was strongly salt-dependent and was approximately a linear function of log N NaCl. An equilibrium shift caused by the counter ion may explain the salt dependence. Hydrogen bonding …
An Investigation Of Solvent Extraction Techniques In The Determination Of Formation Constants Of Complex Species In Aqueous Silver Chloride Solution, David Martin Bodily
An Investigation Of Solvent Extraction Techniques In The Determination Of Formation Constants Of Complex Species In Aqueous Silver Chloride Solution, David Martin Bodily
Theses and Dissertations
Solvent extraction techniques have been used to determine formation constants for molecular silver chloride and dichloro silver (I) ion in unsaturated aqueous solutions. Nitrobenzene was used to extract the molecular silver chloride from the aqueous silver chloride solution. The total concentration of silver in each phase was determined by radioassay techniques and related by an expression involving the equilibrium constants. The reciprocal of the distribution ratio times the activity of the chloride ion was plotted versus the activity of the chloride ion and portions of the curve were analyzed by the method of least squares to obtain values for the …
An Investigation Of The Formation Constant Of Molecular Silver Chloride Using Ion Exchange Techniques.|Nii.|Pan Investigation Of The Rate Of Precipitation Of Silver Sulfide By Thioacetamide, James W. Pyper
Theses and Dissertations
The first part of this work reports on the investigation of the formation constant of molecular silver chloride using ion exchange techniques. In order to determine the formation constant, it was necessary to develop a method of rinsing the sorbed molecular silver chloride from the resin. It was found that desorption with nitrobenzene would remove the sorbed molecular species. However, the formation constant was not calculated since the nitrobenzene used contained hydrogen ion and chloride ion which removed silver ion as well as the molecular species. In the study of the formation of silver sulfide by the reaction between the …
An Investigation Of Complex Species In Silver Chloride Solutions I. Solubility Measurements ; Ii. Ion Exchange Methods, Harry G. Hecht
An Investigation Of Complex Species In Silver Chloride Solutions I. Solubility Measurements ; Ii. Ion Exchange Methods, Harry G. Hecht
Theses and Dissertations
The complex species in silver chloride solutions have been investigated by solubility measurements and by ion exchange methods in this work. The solubility of silver chloride in solutions of varying chloride ion concentrations was determined by radioassay techniques. The method of least squares was used to evaluate the constants for the formation of the aqueous silver chloride and the dichloro-complex species; the values being 1.99 x 103 and 2.98 x 104, respectively, at 25.00°C. Aqueous solver chloride has been shown to be sorbed by Amberlite IR-120 from silver chloride solutions which have an ionic strength of 0.2. This sorption has …