Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Discipline
- Keyword
-
- Barley (2)
- Calorimetry (2)
- DNA (2)
- Volumetric analysis (2)
- Bacillus subtilis (1)
-
- Bibliography (1)
- Cytochrome c. (1)
- Ionizations (1)
- Kidneys (1)
- Lasers (1)
- Little Drum Mountains (1)
- Mercury (1)
- Nuclear reactions (1)
- Nutrition (1)
- Physiology (1)
- Protons (1)
- Rats (1)
- Shoshonitic lavas (1)
- Sulfur hexafluoride (1)
- Swine (1)
- Tetrafluorohydrzine (1)
- Thermochemistry (1)
- Toxicology (1)
- Trace elements in nutrition (1)
- Vitamin B1 deficiency (1)
- Volcano (1)
- Zinc (1)
Articles 1 - 11 of 11
Full-Text Articles in Physical Sciences and Mathematics
State Of Bacterial Dna After Uptake Into The Roots Of Germinating Barley Seeds, Larry Dale Lawson
State Of Bacterial Dna After Uptake Into The Roots Of Germinating Barley Seeds, Larry Dale Lawson
Theses and Dissertations
Germinating barley seeds have been shown to take up Bacillus subtilis DNA labelled with 5-bromouracil and tritiated thymidine and retain the DNA in the root system without substantially degrading it. The percentage of bacterial DNA recovered from the total root DNA was independent of the amount of bacterial DNA available for uptake. Four observations indicated the stability of bacterial DNA after uptake into barley roots: (1) the bacterial DNA retained its transforming ability after recovery from the roots; (2) the labelled B. subtilis DNA showed similar CsCl density gradient patterns before uptake and after uptake; (3) most of the radioactivity …
I. An Isothermal Titration Microcalorimeter. Ii. Importance Of Micronutrients In Nutrition. Iii. Zinc Bibliography, John Willard Gardner
I. An Isothermal Titration Microcalorimeter. Ii. Importance Of Micronutrients In Nutrition. Iii. Zinc Bibliography, John Willard Gardner
Theses and Dissertations
I. An isothermal titration microcalorimeter having a colume of 4 ml and capable of temerature control to ±2 x 10-5°C is described. Major components include a constant temperature water bath controlled to ±3 x 10-4°C, a platinum reation vessel, and an isothermal control circuit consisting of constant Peltier thermoelectric cooling and variable Joule heating controlled by a thermistor in an AC Wheatstone bridge circuit. The calorimeter was tested by measuring the heat of ionization of water and was found to produce data accurate to ± 0.1% where small samples are used such as in the investigation of many biological systems.
|Ni.|Pa Calorimetric Study Of The Heat Of Ionization Of Water At 10 And 40°C, And,|Nii.|Pa Calorimetric Determination Of The Heat Of Reaction Of Tris (Hydroxymethyl) Aminomethane (Tham) With Hydrochloric Acid In Aqueous Solution At 10, 25, And 40°C, Griffith Lyn Kimball
Theses and Dissertations
The heat of neutralization, ΔH_-N, of perchloric acid with sodium hydroxide has been determined at 10 and 40° in a low ionic strength, μ, region using a nonisothermal, constant-temperature-environment solution calorimeter. Correction of the data to infinite dilution by extrapolation of a plot of ΔH_-N vs μ^0.5 gives values at zero ionic strength for the heat of ionization of water of 14.216 and 12.695 kcal/mole, respectively, at 10 and 40° . The data are correlated with those of earlier workers to give an equation allowing the calculation of the heat of ionization of water at zero ionic strength over the …
Little Drum Mountains, An Early Tertiary Shoshonitic Volcanic Center In Millard County, Utah, Stephen H. Leedom
Little Drum Mountains, An Early Tertiary Shoshonitic Volcanic Center In Millard County, Utah, Stephen H. Leedom
Theses and Dissertations
The Little Drum Mountains represent a deeply eroded Eocene-Oligocene volcano, consisting of a vent complex which erupted mafic flows and flow breccias, accompanied by lahars. Flows are dominated by members of the shoshonite suite and contain up to 3.95 percent K2O, mainly occult in K-rich glass, with K2O/Na2O ratios greater than 1.0. In a few interbedded flows, apparently of the calc-alkaline series, pyroxene with varying amounts of plagioclase in a fine-grained groundmass of plagioclase, mafic minerals, and interstitial glass. An ash-flow tuff of the Oligocene Needles Range Formation unconformably overlies the volcanic sequence. Contemporaneous …
Transforming Ability Of Bacillus Subtilis Dna Taken Up By Barley Embryos, Christine Tolman Ence
Transforming Ability Of Bacillus Subtilis Dna Taken Up By Barley Embryos, Christine Tolman Ence
Theses and Dissertations
Purified Bacillus subtilis DNA taken up by one-day-old barley embryos and permanently fixed within the barley cell is shown to have maintained its biological activity in B. subtilis transformation. Cesuim chloride density gradient centrifugation of DNA isolated from the roots of barley embryos after the uptake and incorporation of radioactive, bromouracil-labelled B. subtilis DNA indicates the presence of the bacterial DNA in several discreet bands of well-defined density, whose density is changed by sonication. These preliminary findings concerning the fate of bacterial DNA taken up by barley roots are discussed in terms of the recombination model of Ledoux and Huart …
Reactions Of N₂F₄ And Sf₆ With H₂ Induced By Co₂ Laser Pulses And By Electrical Discharge, John Leslie Lyman
Reactions Of N₂F₄ And Sf₆ With H₂ Induced By Co₂ Laser Pulses And By Electrical Discharge, John Leslie Lyman
Theses and Dissertations
A high power pulsed CO_2 laser was used to induce reactions in gas mixtures containing either N_2F_4 or SF_6. The rate of laser induced dissociation of N_2F_4 to NF_2 was as much as 1000 times faster than the thermal rate, and the rate law was of lower order than the thermal, bimolecular rate law. Explosion threshold and induction time data were obtained for butene stabilized mixtures of N_2F_4 and H_2. HF laser gain was observed during the first few microseconds of CO_2 laser pulse, but it was strongly quenched by the explosion. The laser driven reaction of SF_6 with H_2 …
The Determination Of Thiamin And Its Derivatives In Brain Tissue Of Control, Thiamin-Deficient, Oxythiamin- And Pyrithiamin-Treated Rats, David S. Murdock
The Determination Of Thiamin And Its Derivatives In Brain Tissue Of Control, Thiamin-Deficient, Oxythiamin- And Pyrithiamin-Treated Rats, David S. Murdock
Theses and Dissertations
The determination of total thiamin, free thiamin, thiamin di phosphate (TDP) plus thiamin triphosphate (TTP), and total α- hydroxyethylthiamin (HET) levels in rat brain in control, deficient, oxythiamin- (OTH) and pyrithiamin- (PTH) treated rats was accomplished. It was found that the TDP + TTP/thiamin ratio observed in the thiamin-deficient, OTH- and PTH-treated rats was constant and did not differ from the ratio observed in the control rat brains. The brain levels of TDP + TTP decreased to 39% and 12% of the control thiamin levels in deficient and PTH-treated rats respectively. The brain HET and TDP + TTP levels of …
⁷Li(D,P)⁸Li Excitation Function :|Be[Subscript D] = 0.623 Mev To 1.968 Mev, Albert E. Schilling
⁷Li(D,P)⁸Li Excitation Function :|Be[Subscript D] = 0.623 Mev To 1.968 Mev, Albert E. Schilling
Theses and Dissertations
In this study the excitation function for the ^7Li(d,p)^8Li reaction is determined at incident deuteron energies ranging from 0.623MeV to 1.968MeV by counting the delayed alpha-particles as a result of the ^8Li beta decay. This excitation function reveals resonances at 0.777±0.012MeV and 1.031±0.015MeV with cross sections of 202±9mb and 188±8mb respectively. The uncertainty in the cross section values was due to three independent sources of error within this experiment, the solid angle, the ^8Li half-life, and the target (LiF) thickness. The uncertainty in the energy values was determined from an energy calibration of the accelerator used in this study. Resonances …
The Purification And Characterization Of Cytochrome B₅ From Porcine Kidney, M. Dean Klingler
The Purification And Characterization Of Cytochrome B₅ From Porcine Kidney, M. Dean Klingler
Theses and Dissertations
A soluble form of cytochrome b_5, with a 413/280 nm ratio of nearly unity, has been isolated and purified from porcine kidney using fractional precipitation, ion exchange resins, and gel filtration procedures. The cytochrome b_5 shows heat stability up to 50° and is stable in the pH range 6.0 to 8.5. The oxidized protein exhibits an absorption maximum at 413 nm, while the reduced form shows three peaks: 423, 526, 556 nm. Molecular weight determinations have given variable results, suggesting the possibility of a polymer composed of four and eight units of a 13,500 molecular weight protomer. This study has …
Development Of Standard Procedures For Determination Of [Delta]G° (K), [Delta]H° And [Delta]S° Values Via Potentiometric And Calorimetric Titration Techniques, Jack Hugh Ruckman
Development Of Standard Procedures For Determination Of [Delta]G° (K), [Delta]H° And [Delta]S° Values Via Potentiometric And Calorimetric Titration Techniques, Jack Hugh Ruckman
Theses and Dissertations
Standard computer techniques were developed for computation of ΔG° values from potentiometric titration data and ΔH° values from calorimetric titration data. The procedures developed can be used for any systems having the general equations HA = W^+ + A^- ; H_2A = 2H^+ + A^2- ; and H_3A = 3H^+ + A^3-, where the proton ionizations may be consecutive or simultaneous. The thermodynamic values ΔG°(K), ΔH° and ΔS° were determined for the ionization of protons from the protonated compounds methionine (-NH_3^+ group only), phosphoethanalomine and phosphoserine. The compounds studied were selected because they contain one, two and three ionizable protons …
Thermodynamic Equilibrium Formation Constants And Molal Concentrations Of The Complexed Mercury-Containing Species Of Mercuric Chloride In Added Hydrochloric Acid And Methanol-Water Solvent Mixtures, Karl Jennings Walker
Thermodynamic Equilibrium Formation Constants And Molal Concentrations Of The Complexed Mercury-Containing Species Of Mercuric Chloride In Added Hydrochloric Acid And Methanol-Water Solvent Mixtures, Karl Jennings Walker
Theses and Dissertations
Saturated solutions of mercuric chloride in different methanol-water and hydrochloric acid solvent mixtures were equilibrated at 25.00 ± 0.01°C. Total mercury and free chloride concentrations were determined by analytical procedures and the data were analyzed with the aid of the computer. Tabulated below are the results of this study: [--see thesis for table--]