Physical Sciences and Mathematics Commons^™

Potassium Ion Fluxes In Corneal Epithelial Cells Exposed To Uvb, John L. Ubels, Rachel E. Van Dyken, Julienne R. Louters, Mark P. Schotanus

University Faculty Publications and Creative Works

The goal of this study was to investigate the efflux of K+ from human corneal limbal epithelial cells (HCLE) exposed to ambient levels of UVB, which is known to cause apoptosis, and to examine the effect of K+ channel blockers on loss of potassium induced by UVB. HCLE cells were exposed to 100-200 mJ/cm2 UVB, followed by incubation in culture media with 5.5-100 mM K+, BDS-1, Ba2+ or ouabain. To measure intracellular cations, cells were washed in 280 mM sucrose and lysed in DI water. K+ and Na+ levels in lysates were measured by ion chromatography. HCLE cells showed maximal …

Potassium Ion Fluxes In Corneal Epithelial Cells Exposed To Uvb, John L. Ubels, Rachel E. Van Dyken, Julienne R. Louters, Mark P. Schotanus

University Faculty Publications and Creative Works

The goal of this study was to investigate the efflux of K+ from human corneal limbal epithelial cells (HCLE) exposed to ambient levels of UVB, which is known to cause apoptosis, and to examine the effect of K+ channel blockers on loss of potassium induced by UVB. HCLE cells were exposed to 100-200 mJ/cm2 UVB, followed by incubation in culture media with 5.5-100 mM K+, BDS-1, Ba2+ or ouabain. To measure intracellular cations, cells were washed in 280 mM sucrose and lysed in DI water. K+ and Na+ levels in lysates were measured by ion chromatography. HCLE cells showed maximal …

University Scholar Series: Marc D'Alarcao, Marc D'Alarcao

University Scholar Series

Synthetic Organic Chemistry to Shed Light on Type II Diabetes and Cancer

On March 16, 2011 Marc d'Alarcao spoke in the University Scholar Series hosted by Provost Gerry Selter at the Dr. Martin Luther King, Jr. Library. Marc d'Alarcao is a Professor in the Chemistry Department at SJSU. In this seminar, he gives a progress report on his work aimed at developing and synthesizing new compounds that are designed to take advantage of this metabolic difference. His hope is that these compounds will be selectively toxic to cancer cells and may provide a new strategy for treating this major threat …

Synthesis Of Isatin Derivatives Used For The Inhibition Of Pro-Apoptotic Jurkat T Cells, Charles Michael Clay

Browse all Theses and Dissertations

A range of substituted 1H-indole-2,3-diones (isatins) was synthesized to assess their capability to inhibit caspases and prevent apoptosis in Jurkat T cells. The key steps in the synthesis of such molecules involved electrophilic substitution of the C-5 position of the isatin nucleus (if necessary), N-alkylation, Wolff-Kishner reduction of the C-3 carbonyl group and finally, Knoevenagel condensation. The design and synthesis of such potential inhibitors was guided by SAR studies of peptide based inhibitors such as "Q-VD-O-Ph", as well as small-molecule inhibitors based upon the isatins scaffold. Previously, 3-(2,6-difluorobenzylidene)-5-nitroindolin-2-one has been shown to inhibit apoptosis in human Jurkat T cells at …

Novel Trisubstituted Arylidene Oxindoles With Potent Anti-Apoptotic Properties, Paul J. Repasky

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Apoptosis is a highly complex, multi-step cellular pathway utilized to initiate cell suicide. The family of enzymes largely responsible for the process is the cystienyl aspartic acid proteases (caspases). This study details the design and synthesis of a new class of small molecule caspase inhibitors, namely arylidene oxindoles, which are comparable to the isatin family of inhibitors but which offer the advantage of incorporating three points of variability into the basic scaffold. Moreover, whereas the mode of action of isatins against caspases has been demonstrated to involve nucleophilic addition of the cysteine residue of the enzymes to the C-3 ketone …