Physical Sciences and Mathematics Commons^™

Deglacial And Holocene Environmental Change Recorded In Lake Sediments From The Snowy Mountains, Kosciuszko National Park, Southeastern Australia, Emma J. Mickelson

WWU Graduate School Collection

The sediment record from Lake Albina, the Snowy Mountains, southeastern Australia suggests significant environmental change mostly reflecting shifts in climate from the LGM through the Holocene. Immediately following deglaciation, approximately 19.4 thousand cal ky BP, Lake Albina experienced cold and/or dry conditions with minimal vegetation in the catchment. Cooler conditions persisted with a slight increase in moisture throughout the Antarctic Cold Reversal (ACR; 14.7-13 cal ky BP) aligning closest with a strengthening of the Atlantic Meridional overturning circulation (AMOC) and a northward shift of the South Westerly Winds (SWW). Between 12.9-9.7 cal ky BP, Lake Albina recorded a climate reversal …

Enhancing The Federal Natural Resource Damage Assessment And Restoration Process Through Bayesian Networks: A Case-Study On The Little Mississinewa River, Indiana, April D. Reed

WWU Graduate School Collection

The Federal Natural Resource Damage Assessment and Restoration (NRDAR) program gives Tribes and certain government agencies the authority to assess injury to natural resources and to pursue and implement compensatory action for any resources lost or injured due to unlawful releases of chemicals into the environment. This study was centered around the development of a Bayesian network (BN) decision support tool tailored to the needs of NRDAR practitioners. The goal was to design a probabilistic BN tool that could lend quantitative insight into natural resource injury. A case study was used to develop and demonstrate the tool’s functionality and propriety …

The Biophysical Resilience Capacity Of The Salish Sea’S Tidal Wetlands To Sea Level Rise, Kenna Kuhn

WWU Graduate School Collection

Tidal wetlands offer significant ecosystem services, cultural identity, and economic opportunities, but the impact that projected SLR will have on tidal wetlands in the Salish Sea is not known. In this thesis, I examine the exposure, watershed-scale biophysical resilience capacity, and jurisdictional variation in resilience capacity of the Salish Sea’s tidal wetlands to SLR. I quantify exposure, resilience, and jurisdictional variation using existing spatial data and analysis techniques. I employ a framework for biophysical resilience capacity developed by NOAA and NERRA. This study’s results indicate that there is substantial variation in wetlands area by watershed, from 0 km² to …

Life Cycle Assessment Of A Hemp-Based Thermal Insulation Panel, Kara Davis

WWU Graduate School Collection

Buildings have a monumental impact on the environment and the economy and account for about 40% of energy consumption, greenhouse gas emissions, and materials entering the economy. Life cycle assessment (LCA) is a tool for quantitatively evaluating the environmental impacts of a product throughout its life span commonly applied in the building and construction sector. While past efforts have focused on reducing operational energy and carbon, attention has shifted towards embodied energy and carbon as buildings become more energy efficient. This graduate thesis applies the LCA framework and investigates the life cycle environmental impacts of a hemp-based thermal insulation panel …

Regio- And Diastereoselective Samarium-Mediated Allylic Sulfone Reductions, Cody Schwans

WWU Graduate School Collection

A series of allylic sulfones were synthesized containing a stereodirecting group and chelating element and subjected to samarium diiodide reductions in the presence of a proton donor. The resulting products could be obtained with high regioselectivity (no less than 95:5) and high diastereoselectivity (>10:1) that correlated with the size of the stereodirecting group. A mechanism is proposed that includes loss of the sulfone and formation of a chelated organosamarium intermediate followed by intramolecular protonation by a samarium-bound proton source. In this way, both the regioselectivity and absolute stereochemistry of the resulting products are explained.

Impact Of Riverbank Lupine (Lupinus Rivularis) On Grand Fir (Abies Grandis) Ectomycorrhizal Symbioses, Andrew Labay

WWU Graduate School Collection

Lupine (Lupinus rivularis) is used in the revegetation of coarse sediments surrounding the Elwha basin in Washington State due to its ability to improve soil conditions. Previous research illustrated that seeding lupine with conifers increased growth and foliar nitrogen, however, decreased ectomycorrhizal (ECM) root colonization, an important symbiosis for conifers used in restoration. We hypothesized the observed decrease in ECM may be due to lupine increasing soil nitrogen, negating the need for a fungal symbiont. To investigate this, we explored the interaction between lupine and ECM colonization of grand fir (Abies grandis) in both a field …

Glacial Loss And Threatened Fish: The Future Of Mount Rainier’S Cold-Water Bull Trout Habitats, Kathleen C. Ewen

WWU Graduate School Collection

Glaciers play a key ecological role in the river systems that they support. Cold-water reaches supplied by glacial ice serve as critical habitats for aquatic organisms that rely on specific thermal ranges to survive. Federally threatened Bull Trout (Salvelinus confluentus) require very cold temperatures, like those found in glacial systems, to complete their life cycles. However, glaciers are retreating due to climate change and are expected to continue diminishing throughout this century. Decreased glacial extent could result in warmer stream temperatures downstream from glaciers and, depending on the magnitude of stream temperature increase, cold-water habitats relied upon by Bull Trout …

The Race Toward Carbon Neutral Ecotourism: Leveraging Life Cycle Analysis And Natural Climate Solutions For A Community Adventure Event, Ted Tarricone

WWU Graduate School Collection

Global tourism is an interconnected framework of multiple industries that is influenced by and has impacts on economic, social, and environmental structures. Currently, tourism accounts for roughly 8% of global greenhouse gas emissions, which is expected to grow with industry expansion. To develop a scalable system for ecotourism assessment, emission reduction, and solutions to meet carbon neutrality, a small (n=3894 participants) adventure relay race named Ski to Sea in Bellingham, WA was studied. A life cycle analysis (LCA) conducted on the race showed similar proportional results to other tourism LCAs, where transport made up over 80% of the 325 tonnes …

Quantifying Channel Change Following Post-Fire Debris Flows In A Steep, Coastal Stream, Big Sur, California, Telemak Olsen

WWU Graduate School Collection

Debris flows commonly occur following wildfire in steep landscapes, introducing large volumes of sediment to downstream fluvial systems. Fire-related sediment supply perturbations impact channel morphology, and importantly, fragile aquatic and riparian ecosystems downstream of disturbance. The Big Creek watershed drains 57 km² of steep chaparral and coast redwood forest along California’s Central Coast. Streams in the Big Creek watershed typically exhibit step-pool/cascade morphology and serve as vital spawning habitat for anadromous Steelhead Trout (Oncorhynchus mykiss). In 2020, 97% of the Big Creek watershed burned in the Dolan Wildfire. In January 2021, an atmospheric river event triggered a series of …

Distribution And Mixotrophy Of Cryptophyte Phytoplankton In The Northern Gulf Of Alaska, Megan O'Hara

WWU Graduate School Collection

The Northern Gulf of Alaska (NGA) is a productive subarctic marine ecosystem that supports high abundances of plankton, fishes, seabirds, and mammals. Research has shown that this high productivity is primarily controlled by seasonal and spatial heterogeneity in the lower trophic level food web. Marine cryptophytes are a crucial, yet understudied, phytoplankton group in the NGA. Cryptophytes have the capacity for mixotrophy (acquiring energy through photosynthesis and feeding) which can improve trophic transfer efficiency, increase cellular growth rates, and improve retention of nutrients in the water column. Field samples collected in spring, summer, and fall 2021 surveyed the contribution of …

Contaminants Of Emerging Concern In Puget Sound: Screening, Prioritization, And Estrogenic Mixture Response Assessment, Maya Faber

WWU Graduate School Collection

Contaminants of emerging concern (CECs) are chemicals used in daily life, such as pharmaceuticals, personal hygiene products, steroids, pesticides, and flame retardants. The environmental occurrence and toxicology of CECs are poorly characterized, and they are generally unregulated. Traditional toxicological approaches rely on in vivo methods to test whole organisms for apical endpoints, including survival, reproduction, and growth. This is time-consuming and costly, both financially and in terms of laboratory animal well-being, limiting ecotoxicological data for CECs. To overcome this challenge, we are utilizing alternative approaches, including New Approach Methodologies (NAMs), to perform a screening-level evaluation of CECs present in Puget …

Class A Sortases: Structures And Alternative Substrate Binding And Cleavage, Brandon Vogel

WWU Graduate School Collection

Sortases, consisting of classes A-F, are cysteine transpeptidases found in the cell wall of Gram-positive bacteria. They play a crucial role in ligating proteins to the cell wall that are responsible for cell adhesion, immune evasion, host cell invasion, and nutrient acquisition through a transpeptidation reaction. Consequently, they are an attractive therapeutic target. Class A sortases are also utilized in protein engineering applications such as sortase-mediated ligations and sortagging. Despite extensive research in the past two decades, gaps persist in understanding how class A sortases recognize their substrates, primarily due to a lack of structural information on sortases non-covalently bound …

Using Chemical Zoning In Minerals To Understand Magmatic Processes At Augustine Volcano, Alaska, Sloane Kennedy

WWU Graduate School Collection

The eruption behavior of a volcano is intrinsically linked to magmatic evolution. Augustine Volcano is an active stratovolcano in the Aleutian Arc (AK, USA), showing stratigraphic evidence that eruptions before 1,800 C.E. were more explosive, producing thick pumice deposits, compared to the six eruptions observed in the last 200 years. Tephra B, an understudied ~400-year-old pumice fall unit, represents the last more explosive style eruption at Augustine - making it ideal for studying how changes in magmatic evolution can result in changes in eruption style. For this thesis, I used textural zoning patterns and chemical variations (major and trace elements) …

A Characterization Of Hyporheic Temperatures With Applications For Salmon Habitat Restoration In A Thermally Impaired River, Sydney Jantsch

WWU Graduate School Collection

This thesis project is part of an ongoing study assessing the effectiveness of a potentially innovative habitat restoration strategy for Pacific salmon in thermally impaired rivers. This strategy uses engineered log jams (ELJs) to create pockets of cool-water refuge by forming deep scour pools and promoting localized upwellings of shallow subsurface (i.e., hyporheic) water. This project seeks to characterize the relationship between hyporheic temperature and overlying surface stream temperature to elucidate the extent to which hyporheic upwellings can deliver cool water to ELJ-formed pools during the summer low-flow season. Among six sites within a 2.7 km-long study reach on the …

Kinetic Analysis Of The Grafting Thermal Ring-Opening Polymerization Reaction Of Benzoxazine With Sulfonyl-Ester Functionalized Polymers, Tawakalt Adetoun Akinjobi

WWU Graduate School Collection

This project will explore the fundamental mechanistic details and kinetics of a grafting polymerization reaction that occurs with blended benzoxazine monomers (or resins) and polymers functionalized with reactive sulfonyl-ester leaving groups [such as tosylate (Ts), nosylate (Ns) or mesylate (Ms) groups]. Isoconversional analysis (ICA) is one of the most important methods for establishing the kinetics of the complex reactions associated with curing thermosets. In the case of ICA, activation energy is measured at fixed values of reaction conversion.²⁶ For thermosets the data are typically based on differential scanning calorimetry (DSC). The combinatorial approach not only provides uncertainty associated with …

A Mixed-Methods Study Of Geoscience Identity, Race/Ethnicity, And Gender In Senior Undergraduate Geoscience Majors, Willa Rowan

WWU Graduate School Collection

I conducted a mixed methods study of geoscience identity in undergraduate students to examine the cultural and social aspects of geoscience degree programs. White students are overrepresented in geoscience, and a priority of anti-racism efforts in the field is listening to the experiences of students who are Black, Indigenous, or people of color (BIPOC) to better inform equity and inclusion goals. Structural racism in geoscience pushes BIPOC students out of the field, and it can be better understood by studying socially constructed aspects of learning such as geoscience identity. This study is the first to measure geoscience identity with a …

Biomarker Study Of Bellingham Bay : Identifying How Urbanization Has Affected Carbon Storage And Eelgrass, Jess Shulman

WWU Graduate School Collection

Understanding sediment sources and fluxes throughout coastal zones is essential to evaluate shoreline stability, ecosystem health, and the potential for carbon storage. In Bellingham Bay, WA, like many developed coastal settings, urban areas have replaced forested cover and altered sediment fluxes, yet little is known of their offshore impacts. I analyzed n-alkanes, found in plant leaf waxes preserved in marine sediments of Bellingham Bay to characterize sediment sources and reconstruct changes in the relative contributions of eelgrass beds to sedimentary organic matter since pre-industrial times using a linear mixing model. Eight 2-meter-long cores were analyzed in order to determine how …

U-Pb Zircon Geochronology And Structure Of Regional Blueschist Units In The Easton Metamorphic Suite, Northwest Cascades, Wa, Katherine Lang

WWU Graduate School Collection

The Easton metamorphic suite of the Northwest Cascades Thrust System (NWCS) is a well-preserved subduction accretion complex in Washington State. The regional blueschist units of the Easton metamorphic suite include the Mt. Josephine semi-schist, Darrington Phyllite, and Shuksan greenschist/blueschist and all are interpreted to have accreted after the onset of Jurassic subduction beneath North America. This study uses zircon U-Pb geochronology, structure, and field observations to test the regional correlations between units in the Easton metamorphic suite and address models for the timing of subduction accretion along the North American margin in the Late Jurassic – Early Cretaceous. The results …

Modeling The Effects Of Projected Climate Warming On Stream Temperatures In The Stillaguamish River Basin, Emily Esther Gebheim Smoot

WWU Graduate School Collection

The Stillaguamish River is a snow-and-rain mixed basin and the fifth largest river in the Puget Sound basin. Elevations in the 1700 km² Stillaguamish River basin reach roughly 2000 m and historically a snowpack is sustained above 1000 m. Snowmelt in the basin is important for sustaining spring and summer streamflow and buffering stream temperatures. Stream temperature increases are of significant concern because of the threatened Chinook salmon (Oncorhynchus tshawytscha) population.

I reexamined projected stream temperatures in the Stillaguamish River by forcing the coupled Distributed Hydrology Soil Vegetation Model and River Basin Model with dynamically downscaled meteorological …

Reconstructing Wildfire Regime During The Warm Paleocene-Eocene Climate Using Molecular Biomarkers From The Chuckanut Formation In Northwest Washington, U.S.A., Alexandra Elise Thompson

WWU Graduate School Collection

Wildfires are expected to increase in frequency and severity as climate changes in the Pacific Northwest of North America. To better understand the effect of warming climate on wildfire regimes, I present the first reconstruction of past wildfire frequency and severity during the Paleocene-Eocene transition in northwest Washington state, U.S.A. The Chuckanut Formation is a late Paleocene to Eocene sedimentary unit with a robust paleobotanical record showing the existence of subtropical forests in northwest Washington during the Paleocene, transitioning to a relatively cooler Eocene, dominated by temperate mixed forests. Geochemical records of environmental change coinciding with Paleocene-Eocene climatic change in …

Value Mapping And The Community Wildfire Protection Planning Process In Entiat, Wa, Katharine Kiendl

WWU Graduate School Collection

Community Wildfire Protection Plans (CWPP) offer benefits for communities effected by wildfire risk and ignitions. CWPP’s serve several functions; they develop interlocal agreements between agencies that provide wildfire response and management, they develop hazard mitigation and wildfire education programs, and they define areas as wildland urban interface (WUI). The CWPP drafting process can impact the resources available to a wildfire risk community. However, despite the impact to communities effected by wildfire, CWPP planning is considered a categorical exclusion under the National Environmental Policy Act (NEPA) allowing CWPP’s to be developed without public review. Given that community plans with public participation …

The Economic And Emissions Reduction Potential Of Air Source Heat Pumps As A Replacement For Natural Gas And Electric Resistance Space Heating In The Contiguous United States, Joshua Schraer

WWU Graduate School Collection

It is widely believed that to reduce CO₂ emissions the best strategy is to electrify everything, decarbonize the grid, and improve energy efficiency. This research looks specifically at the use of air source heat pumps (AHP) as a tool to reduce the CO₂ emissions of heating energy in the residential sector. The landscape of residential energy use is complicated by a broad range of factors. We compare AHP, natural gas (NG), and electric resistance (ER) heating using data from energy prices, temperature, appliance efficiency, building efficiency and marginal emissions data from 2019 as well as modeled data of …

Activity And Selectivity Of Class B Sortase Enzymes, Sophie Jackson

WWU Graduate School Collection

Gram-positive bacteria attach many proteins to their cell walls via sortase enzymes. Sortases are cysteine transpeptidases and are grouped into 6 classes, A-F. Sortase enzymes, particularly sortase A from Staphylococcus aureus, have been used extensively for in vitro protein ligations. Here, we investigate substrate-binding in sortase A from Streptococcus pyogenes. In addition, class B sortases are typically overlooked for research and development due to low in vitro activity and incomplete knowledge of substrate specificity. Here, we investigate the activity of class B sortases from Bacillus anthracis (baSrtB), Clostridioides difficile (cdSrtB), Listeria monocytogenes (lmSrtB), and Staphylococcus aureus (saSrtB). Of these, …

Selectivity And Structure Of Chimeric Loop Swaps In Sh2 Domains, Sarah N. Smith

WWU Graduate School Collection

SH2 (Src Homology 2) domains are protein domains that bind to phosphorylated tyrosine residues within cell signaling cascades. They have been found to play a role in certain cancers and immunological disorders. Despite their importance in cell signaling and medical relevance, the structural basis of the various selectivity classes of SH2 domains is only partially understood. Previous research found that the EF and BG loops of the domains contribute to forming the peptide binding pocket, and thus impact their selectivity. To further understand the role of these loops in selectivity, we engineered chimeric SH2 domains by swapping the EF and …

New Synthetic Methods Based On Silicon-Tethered Nucleophilic Addition Reactions, Alexie W. Clover

WWU Graduate School Collection

With the recent discovery of an iodine mediated rearrangement of diallylsilanes, we set out to investigate a similar fluorine mediated rearrangement, aimed at introducing a new method for synthesizing organofluorine compounds. Interest in incorporating fluorine into organic molecules has grown significantly in recent years, primarily for medicinal applications. Since certain fluorination methods require the use of mCPBA, a common epoxidizing reagent, control experiments were performed on the reaction of several diallylsilanes with mCPBA, anticipating that a competing epoxidation of the diallylsilanes might occur. It was found that the formation of the hydroxy ester occurred through a regioselective epoxide opening from …

Tuned Gold Layer Growth Onto Plasmonic Sensing Silver Nanocubes Via Synthetic Control Of Reduction Potentials., Nicolas Hall

WWU Graduate School Collection

Metallic nanoparticles (mNPs) are commonly employed as sensors and detection tools due to their unique plasmonic properties. Silver NPs exhibit these properties in heightened capacity in comparison to other metals. However, Ag NPs are susceptible to oxidation, degradation over time and are biotoxic. These issues are commonly addressed by creating Ag-alloy NPs or by adding additional layers to Ag NPs. This work improves upon these methods by focusing on the growth of an Au layer onto Ag nanocubes (AgNCs), resulting in a layered Au-Ag NC (Au@AgNC). The resulting morphology of these Au@AgNCs are dependent on the synthetic pathway taken and …

Evaluating Leaf Trait Variation In High Elevation Bristlecone Pine (Pinus Longaeva) Under Increasing Water Stress: Insights From Needle Length, Stomatal Density, And Cambial Growth, Audrey Salerno

WWU Graduate School Collection

Increasing aridification caused by climate change is altering growth patterns in trees. There is revived attention on how foliar traits respond to climate and the relationship of these traits to ring width. Bristlecone pine (Pinus longaeva, DK Bailey), a long-lived conifer found at high elevations in the cool and dry intermountain west of America, is used in paleoclimate reconstructions by measurement of their annually resolvable tree rings. The species also has annually datable needles retained on their branches for an average of 45 years making it the ideal subject for research on foliar trait and growth relationships under …

Connections Between Eruption Style And Magmatic Reservoir Evolution: Insights From Augustine Volcano, Alaska, Usa, Mahinaokalani G. Robbins

WWU Graduate School Collection

At a single volcano, eruptive behavior can change through time as the plumbing system evolves. Augustine Volcano, a frequently active intermediate stratovolcano in the Alaska-Aleutian arc (USA), is an ideal setting to investigate magma reservoir processes due to its frequent modern and Holocene eruption history. Its most recent 2006 CE eruption included mixed effusive and moderately explosive (VEI 3) events and has been studied in detail. Proximal fall deposits from this eruption were generally mixed fine ash to lapilli with variable thickness, but typically ~5 cm on the island (Wallace et al., 2010). The Pre-contact “Tephra M” was deposited by …

Using Qm/Mm Methods To Explore Sortase Enzyme Intermediates, Kinetics, And Stability, Kyle Whitham

WWU Graduate School Collection

Biochemistry has seen advancements in methods and understanding of the inner workings of proteins, yet biochemists struggle to see real time reaction pathways of protein intermediates. This is where computational chemistry comes in and fills in the holes in knowledge through the use of Quantum Mechanical (QM) models. QM chemistry alone does not give results in a reasonable timescale to predict protein chemistry in a reasonable amount of time. Computational chemistry methods such as Quantum mechanical (QM)/ Molecular Mechanical (MM) (QM/MM), allow us to split the in-silico system into two regions that utilize a fast MM force field region and …

Modeling 21st Century Peak Flows In The Nooksack River Basin In Northwestern Washington State Using Dynamically-Downscaled Global Climate Model Projections, Evan A. Paul

WWU Graduate School Collection

The Nooksack River in northwestern Washington State provides freshwater for agriculture, municipal, and industrial use and serves as a vital habitat for endangered salmon, a resource that is of cultural and economic importance to the Nooksack Indian Tribe and the surrounding region. As more landscape becomes exposed to rain rather than snow and heavy winter precipitation events intensify (i.e., atmospheric rivers), peak flows and sediment delivery to streams will increase due to rapid runoff, resulting in salmon habitat degradation and increased flood risk. Thus, anticipating the effect of climate change on peak flows is crucial for salmon habitat restoration efforts …