Physical Sciences and Mathematics Commons^™

On The Mechanism Of Rna Phosphodiester Backbone Cleavage In The Absence Of Solvent, Christian Riml, Heidelinde Glasner, M T. Rodgers, Ronald Micura, Kathrin Breuker

Chemistry Faculty Research Publications

Ribonucleic acid (RNA) modifications play an important role in the regulation of gene expression and the development of RNA-based therapeutics, but their identification, localization and relative quantitation by conventional biochemical methods can be quite challenging. As a promising alternative, mass spectrometry (MS) based approaches that involve RNA dissociation in ‘top-down’ strategies are currently being developed. For this purpose, it is essential to understand the dissociation mechanisms of unmodified and posttranscriptionally or synthetically modified RNA. Here, we have studied the effect of select nucleobase, ribose and backbone modifications on phosphodiester bond cleavage in collisionally activated dissociation (CAD) of positively and negatively …