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University of Nebraska - Lincoln

United States Geological Survey: Staff Publications

2007

Microsatellites

Articles 1 - 2 of 2

Full-Text Articles in Physical Sciences and Mathematics

Microsatellite Loci For Distinguishing Spotted Owls (Strix Occidentalis), Barred Owls (Strix Varia), And Their Hybrids, W. Chris Funk, Thomas D. Mullins, Eric D. Forsman, Susan M. Haig Jan 2007

Microsatellite Loci For Distinguishing Spotted Owls (Strix Occidentalis), Barred Owls (Strix Varia), And Their Hybrids, W. Chris Funk, Thomas D. Mullins, Eric D. Forsman, Susan M. Haig

United States Geological Survey: Staff Publications

We identified four diagnostic microsatellite loci that distinguish spotted owls (Strix occidentalis), barred owls (Strix varia), F1 hybrids and backcrosses. Thirty-four out of 52 loci tested (65.4%) successfully amplified, and four of these loci (11.8%) had allele sizes that did not overlap between spotted and barred owls. The probability of correctly identifying a backcross with these four loci is 0.875. Genotyping potential hybrid owls with these markers revealed that field identifications were often wrong. Given the difficulty of identifying hybrids in the field, these markers will be useful for hybrid identification, law enforcement and spotted …


Conservation Genetics Of Snowy Plovers (Charadrius Alexandrinus) In The Western Hemisphere: Population Genetic Structure And Delineation Of Subspecies, W. Chris Funk, Thomas D. Mullins, Susan M. Haig Jan 2007

Conservation Genetics Of Snowy Plovers (Charadrius Alexandrinus) In The Western Hemisphere: Population Genetic Structure And Delineation Of Subspecies, W. Chris Funk, Thomas D. Mullins, Susan M. Haig

United States Geological Survey: Staff Publications

We examined the genetic structure of snowy plovers (Charadrius alexandrinus) in North America, the Caribbean, and the west coast of South America to quantify variation within and among breeding areas and to test the validity of three previously recognized subspecies. Sequences (676 bp) from domains I and II of the mitochondrial control region were analyzed for 166 snowy plovers from 20 breeding areas. Variation was also examined at 10 microsatellite loci for 144 snowy plovers from 14 breeding areas. The mtDNA and microsatellite data provided strong evidence that the Puerto Rican breeding group is genetically divergent from sites …