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Saprolegniaceae Identified On Amphibian Eggs Throughout The Pacific Northwest, Usa, By Internal Transcribed Spacer Sequences And Phylogenetic Analysis, Jill E. Petrisko, Christopher A. Pearl, David S. Pilliod, Peter P. Sheridan, Charles F. Williams, Charles R. Peterson, R. Bruce Bury

United States Geological Survey: Staff Publications

We assessed the diversity and phylogeny of Saprolegniaceae on amphibian eggs from the Pacific Northwest, with particular focus on Saprolegnia ferax, a species implicated in high egg mortality. We identified isolates from eggs of six amphibians with the internal transcribed spacer (ITS) and 5.8S gene regions and BLAST of the GenBank database. We identified 68 sequences as Saprolegniaceae and 43 sequences as true fungi from at least nine genera. Our phylogenetic analysis of the Saprolegniaceae included isolates within the genera Saprolegnia, Achlya and Leptolegnia. Our phylogeny grouped S. semihypogyna with Achlya rather than with the Saprolegnia reference …